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KD/KO Validated

RIOK3 Polyclonal antibody

RIOK3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 13593-1-AP

Publications(8)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

EC:2.7.11.1, RIO kinase 3, RIO kinase 3 (yeast), Serine/threonine-protein kinase RIO3, SUDD

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIPIHCIF/ICC

WB analysis using 13593-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13593-1-AP (RIOK3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse testis using 13593-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 13593-1-AP (RIOK3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of MCF-7 using 13593-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 13593-1-AP (RIOK3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 13593-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13593-1-AP (RIOK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of mouse testis using 13593-1-AP
IP result of anti-RIOK3 (IP:13593-1-AP, 4ug; Detection:13593-1-AP 1:600) with mouse testis tissue lysate 4000ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human breast cancer using 13593-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13593-1-AP (RIOK3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human breast cancer using 13593-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13593-1-AP (RIOK3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of MCF-7 using 13593-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using RIOK3 antibody (13593-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of A431 using 13593-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using RIOK3 antibody (13593-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Publications (8)
Protocols
Reviews (1)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HEK-293T cells, mouse testis tissue, MCF-7 cells, rat testis tissue, HeLa cells, Jurkat cells
Positive IP detected in	mouse testis tissue
Positive IHC detected in	human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells, A431 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:5000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13593-1-AP targets RIOK3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag4497 Product name: Recombinant human RIOK3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 215-516 aa of BC039729 Sequence: RRSARLHEKKEHSTAEKAVDPKTRLLMYKMVNSGMLETITGCISTGKESVVFHAYGGSMEDEKEDSKVIPTECAIKVFKTTLNEFKNRDKYIKDDFRFKDRFSKLNPRKIIRMWAEKEMHNLARMQRAGIPCPTVVLLKKHILVMSFIGHDQVPAPKLKEVKLNSEEMKEAYYQTLHLMRQLYHECTLVHADLSEYNMLWHAGKVWLIDVSQSVEPTHPHGLEFLFRDCRNVSQKGGVKEALSERELFNAVSGLNITADNEADFLAEIEALEKMNEDHVQKNGRKAASFLKDDGDPPLLYDE Predict reactive species
Full Name	RIO kinase 3 (yeast)
Calculated Molecular Weight	516 aa, 60 kDa
Observed Molecular Weight	60 kDa
GenBank Accession Number	BC039729
Gene Symbol	RIOK3
Gene ID (NCBI)	8780
RRID	AB_2178105
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O14730
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RIOK3(Serine/threonine-protein kinase RIO3) is initially characterized as a homolog of Aspergillus nidulans sudD and showed down-regulation at the invasive front of malignant melanomas.On Western blots, human RIOK3 migrated as a 60-kD protein. Northern blot analysis indicated that the approximately 4-kb RIOK3 mRNA is expressed widely in human tissues, with additional smaller transcripts present in testis.

Protocols

Product Specific Protocols
IF protocol for RIOK3 antibody 13593-1-AP	Download protocol
IHC protocol for RIOK3 antibody 13593-1-AP	Download protocol
IP protocol for RIOK3 antibody 13593-1-AP	Download protocol
WB protocol for RIOK3 antibody 13593-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

Species	Application	Title
human	WB	Mol Cell Altered m6A Modification of Specific Cellular Transcripts Affects Flaviviridae Infection. Authors - Nandan S Gokhale View Article
mouse	WB	Genes Dev miR-191 regulates mouse erythroblast enucleation by down-regulating Riok3 and Mxi1. Authors - Lingbo Zhang View Article
human	WB	Biomedicines RIOK2 Inhibitor NSC139021 Exerts Anti-Tumor Effects on Glioblastoma via Inducing Skp2-Mediated Cell Cycle Arrest and Apoptosis. Authors - Min Yu View Article
mouse	WB	Cell Rep Riok3 inhibits the antiviral immune response by facilitating TRIM40-mediated RIG-I and MDA5 degradation. Authors - Yong Shen KO Validated View Article
		Life Sci Alliance K48- and K63-linked ubiquitin chain interactome reveals branch- and length-specific ubiquitin interactors Authors - Anita Waltho View Article
human	WB	Mol Cell The integrated stress response regulates 18S nonfunctional rRNA decay in mammals Authors - Aaztli R Coria View Article