Tested Applications
| Positive WB detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13724-1-AP targets EPHA8 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4503 Product name: Recombinant human EPHA8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 35-350 aa of BC038796 Sequence: LDTSTIHGDWGWLTYPAHGWDSINEVDESFQPIHTYQVCNVMSPNQNNWLRTSWVPRDGARRVYAEIKFTLRDCNSMPGVLGTCKETFNLYYLESDRDLGASTQESQFLKIDTIAADESFTGADLGVRRLKLNTEVRSVGPLSKRGFYLAFQDIGACLAILSLRIYYKKCPAMVRNLAAFSEAVTGADSSSLVEVRGQCVRHSEERDTPKMYCSAEGEWLVPIGKCVCSAGYEERRDACVACELGFYKSAPGDQLCARCPPHSHSAAPAAQACHCDLSYYRAALDPPSSACTRPPSAPVNLISSVNGTSVTLEWAP Predict reactive species |
| Full Name | EPH receptor A8 |
| Calculated Molecular Weight | 1005 aa, 111 kDa |
| Observed Molecular Weight | 120 kDa |
| GenBank Accession Number | BC038796 |
| Gene Symbol | EPHA8 |
| Gene ID (NCBI) | 2046 |
| RRID | AB_10638906 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P29322 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for EPHA8 antibody 13724-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The EPHA8 Polyclonal antibody (Cat# 13724-1-AP) has 1 citation. It was cited in Oncology Reports in a 2021 study investigating EphA8's role in inhibiting cell apoptosis via AKT signaling and its association with poor prognosis in breast cancer. The research field is oncology, specifically breast cancer biology and apoptotic signaling pathways. The antibody was used for IHC and WB applications in human samples.
| Species | Application | Title |
|---|---|---|
Oncol Rep EphA8 inhibits cell apoptosis via AKT signaling and is associated with poor prognosis in breast cancer. |
