Tested Applications
| Positive WB detected in | MCF-7 cells, human kidney tissue, mouse stomach tisssue |
| Positive IHC detected in | human stomach cancer tissue, human colon cancer tissue, human ovary tumor tissue, human stomach tissue, mouse stomach tissue, rat stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13734-1-AP targets TFF1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4541 Product name: Recombinant human TFF1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-84 aa of BC032811 Sequence: MATMENKVICALVLVSMLALGTLAEAQTETCTVAPRERQNCGFPGVTPSQCANKGCCFDDTVRGVPWCFYPNTIDVPPEEECEF Predict reactive species |
| Full Name | trefoil factor 1 |
| Calculated Molecular Weight | 84 aa, 9 kDa |
| Observed Molecular Weight | 9-12 kDa |
| GenBank Accession Number | BC032811 |
| Gene Symbol | TFF1 |
| Gene ID (NCBI) | 7031 |
| RRID | AB_2202853 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P04155 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TFF1 (Trefoil factor 1, formerly known as pS2) is a small-secreted protein that belongs to the family of trefoil peptides, and characterized by the presence of one to six cysteine-rich P domains. TFF1 expression is frequently lost in gastric carcinoma, probably through mechanism of DNA methylation, and it is therefore considered as a tumor suppressor gene. The function of TFF1 was first controversially considered as an oncogene in breast cancer.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TFF1 antibody 13734-1-AP | Download protocol |
| IHC protocol for TFF1 antibody 13734-1-AP | Download protocol |
| WB protocol for TFF1 antibody 13734-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TFF1 antibody (Cat# 13734-1-AP) has 17 citations published in journals including Cell Death Differentiation, J Exp Med, Phytomedicine, Cell Death Discovery, JCI Insight, Cells, Translational Oncology, Biomed Pharmacother, Bone, J Virol, Neuropeptides, and others. Research fields span breast cancer, gastric cancer, colorectal cancer, ovarian cancer, chromatin regulation, EMT, bone metastasis, gastric mucosal injury, and intestinal physiology. Applications include WB, IHC, and IF in human, mouse, and rat samples.
| Species | Application | Title |
|---|---|---|
Cell Death Differ BAP18 facilitates CTCF-mediated chromatin accessible to regulate enhancer activity in breast cancer | ||
J Exp Med Single-cell transcriptome analysis reveals differential nutrient absorption functions in human intestine. | ||
Phytomedicine Yiqi Huayu Jiedu formula inhibits JAK2/STAT3-mediated partial EMT in treating chronic atrophic gastritis | ||
Cell Death Discov A novel chemical inhibitor suppresses breast cancer cell growth and metastasis through inhibiting HPIP oncoprotein. | ||
Cell Mol Gastroenterol Hepatol Novel Acinar Metaplastic States Uncovered in Exocrine Pancreas Disease. |
