Tested Applications
| Positive WB detected in | HEK-293 cells, human brain tissue, human testis tissue, mouse skeletal muscle tissue, PC-3 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human oesophagus cancer tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13779-1-AP targets DZIP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4772 Product name: Recombinant human DZIP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 496-867 aa of BC041804 Sequence: FSSHILEPIEELSEEEKGRENEQKLNNNKMHLRKALKSNSSLTKGLRTMVEQNLMEKLETLGINADIRGISSDQLHRVLKSVESERHKQEREIPNFHQIREFLEHQVSCKIEEKALLSSDQCSVSQMDTLSTGEVPKMIQLPSKNRQLIRQKAVSTDRTSVPKIKKNVMEDPFPRKSSTITTPPFSSEEEQEDDDLIRAYASPGPLPVPPPQNKGSFGKNTVKSDADGTEGSEIEDTDDSPKPAGVAVKTPTEKVEKMFPHRKNVNKPVGGTNVPEMFIKKEELQELKCADVEDEDWDISSLEEEISLGKKSGKEQKEPPPAKNEPHFAHVLNAWGAFNPKGPKGEGLQENESSTLKSSLVTVTDWSDTSDV Predict reactive species |
| Full Name | DAZ interacting protein 1 |
| Calculated Molecular Weight | 867 aa, 98 kDa |
| Observed Molecular Weight | 100-113 kDa |
| GenBank Accession Number | BC041804 |
| Gene Symbol | DZIP1 |
| Gene ID (NCBI) | 22873 |
| RRID | AB_2096927 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86YF9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DZIP1, also named as DZIP, DZIP2 and KIAA0996, belongs to the DZIP C2H2-type zinc-finger protein family. DZIP1 has been reported to be associated with centrosomes and ciliogenesis in cells (PMID: 32051257). DZIP1 may participate in spermatogenesis via its interaction with DAZ1, and play a role in primary formation (PMID: 15081113, 19852954). DZIP1 has 3 isoforms with the molecular mass of 31, 97 and 99 kDa. It may be a potential therapeutic target of oral squamous carcinoma (PMID: 31450126).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for DZIP1 antibody 13779-1-AP | Download protocol |
| IHC protocol for DZIP1 antibody 13779-1-AP | Download protocol |
| IP protocol for DZIP1 antibody 13779-1-AP | Download protocol |
| WB protocol for DZIP1 antibody 13779-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The DZIP1 Polyclonal antibody (Cat# 13779-1-AP) has 3 citations published in Nature Genetics, Developmental Biology, and Developmental Dynamics. The associated research fields include ciliary transition zone biology and autosomal recessive polycystic kidney disease (DZIP1L mutations), Joubert syndrome with ciliary membrane extension and length regulation (Arl13b function), and mammalian cardiac valve development via the Cby1-β-catenin signaling mechanism.
| Species | Application | Title |
|---|---|---|
Nat Genet Mutations in DZIP1L, which encodes a ciliary-transition-zone protein, cause autosomal recessive polycystic kidney disease. | ||
Dev Biol A function for the Joubert syndrome protein Arl13b in ciliary membrane extension and ciliary length regulation. | ||
Dev Dyn DZIP1 regulates mammalian cardiac valve development through a Cby1-β-catenin mechanism.
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