Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human pancreas cancer tissue, human urothelial carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13847-1-AP targets ACSL6 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4817 Product name: Recombinant human ACSL6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 48-349 aa of BC047453 Sequence: THYYDDARTMYQVFRRGLSISGNGPCLGFRKPKQPYQWLSYQEVADRAEFLGSGLLQHNCKACTDQFIGVFAQNRPEWIIVELACYTYSMVVVPLYDTLGPGAIRYIINTADISTVIVDKPQKAVLLLEHVERKETPGLKLIILMDPFEEALKERGQKCGVVIKSMQAVEDCGQANHQAPVPPQPDDLSIVCFTSGTTGNPKGAMLTHGNVVADFSGFLKVTEGDIRLLSDDMKALCPTIFPVVPRLLNRMYDKIFSQANTPLKRWLLEFAAKRKQAEVRSGIIRNDSIWDELFFNKIQASL Predict reactive species |
| Full Name | acyl-CoA synthetase long-chain family member 6 |
| Calculated Molecular Weight | 78 kDa |
| Observed Molecular Weight | 74 kDa |
| GenBank Accession Number | BC047453 |
| Gene Symbol | ACSL6 |
| Gene ID (NCBI) | 23305 |
| RRID | AB_3741844 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UKU0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ACSL6 (Acyl-CoA Synthetase Long Chain Family Member 6) is a long-chain lipoyl-coenzyme A synthetase that is mainly responsible for catalyzing the binding of long-chain fatty acids to coenzyme A to form acyl-coenzyme A (Acyl-CoA). ACSL6 is involved in fatty acid metabolic transporter and the synthesis of cell membrane phospholipids, which affects the cell structure and function. ACSL6 plays an extremely important role in the pathogenesis of cancers such as hepatocellular carcinoma, lung cancer and neuroblastoma, suggesting that it may serve as a biomarker and therapeutic target for cancer therapy.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ACSL6 antibody 13847-1-AP | Download protocol |
| IP protocol for ACSL6 antibody 13847-1-AP | Download protocol |
| WB protocol for ACSL6 antibody 13847-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
| Species | Application | Title |
|---|---|---|
Life Sci ACSL6 alleviates myocardial hypertrophy by ameliorating cardiac lipid synthesis and mitochondrial function. | ||
Int J Biol Macromol Structural and functional characterization of Acyl-CoA Synthetase Long-Chain Family Member 3 (ACSL3) and its targeted inhibition by gramicidin in multiple myeloma. | ||
J Ethnopharmacol Lingguizhugan decoction improves cognitive impairment in APP/PS1 mice by promoting meningeal lymphatic drainage based on fatty acid degradation pathway. | ||
Mol Biol Rep Impaired lipolysis and mitochondrial dysfunction in the epididymal tissues of high-fat diet-fed mice. |
