Tested Applications
| Positive WB detected in | HL-60 cells, HeLa cells, HepG2 cells |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13954-1-AP targets CHEK2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5065 Product name: Recombinant human CHEK2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC004207 Sequence: MSRESDVEAQQSHGSSACSQPHGSVTQSQGSSSQSQGISSSSTSTMPNSSQSSHSSSGTLSSLETVSTQELYSIPEDQEPEDQEPEEPTPAPWARLWALQDGFANLECVNDNYWFGRDKSCEYCFDEPLLKRTDKYRTYSKKHFRIFREVGPKNSYIAYIEDHSGNGTFVNTELVGKGKRRPLNNNSEIALSLSRNKVFVFFDLTVDDQSVYPKALRDEYIMSKTLGSGACGEVKLAFERKTCKKVAIKIISKRKFAIGSAREADPALNVETEIEILKKLNHPCIIKIKNFFDAEDYYIVLELMEGGELFDKVVGNKRLKEATCKLYFYQMLLAVQYLHENGIIHRDLKPE Predict reactive species |
| Full Name | CHK2 checkpoint homolog (S. pombe) |
| Calculated Molecular Weight | 61 kDa |
| Observed Molecular Weight | 65 kDa |
| GenBank Accession Number | BC004207 |
| Gene Symbol | CHEK2 |
| Gene ID (NCBI) | 11200 |
| RRID | AB_10598159 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O96017 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Chk2(Checkpoint kinase 2) is also named as CDS1, CHEK2, RAD53 and belongs to the CHK2 subfamily. It is a protein kinase that is activated in response to DNA damage and involved in cell cycle arrest. It also mediates the induction of senescence in fibroblasts, but is dispensable for the induction of telomere dysfunction checkpoints at the stem and progenitor cell level(PMID:20577265). This protein can exsit as a dimer (PMID:19782031). Active forms of CHEK2 devoid of phosphorylation at Thr-68 can form unstable dimers(PMID:12386164). Defects in CHEK2 is a cause of susceptibility to breast cancer (BC)(PMID:21618645). It has 12 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CHEK2 antibody 13954-1-AP | Download protocol |
| IP protocol for CHEK2 antibody 13954-1-AP | Download protocol |
| WB protocol for CHEK2 antibody 13954-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CHEK2 Polyclonal antibody (Cat# 13954-1-AP) has 62 citations across journals including Nature Communications, Cancer Research, Cell Death & Differentiation, Oncogene, EMBO Reports, PLoS Biology, EBioMedicine, and Science Reports. Research fields span DNA damage repair, cancer biology (lung, breast, ovarian, renal, esophageal, and pancreatic cancers), immunology, cellular senescence, toxicology, neurodegeneration, and infectious disease. The antibody was primarily used for Western blot, with some IHC, IF, and Co-IP applications across human, mouse, and rat species.
| Species | Application | Title |
|---|---|---|
Cancer Res Adaptive Regulation of dNTP Homeostasis Confers Osimertinib Resistance in EGFR-Mutant Non-small Cell Lung Carcinoma. | ||
Nat Commun DNA-PK-mediated phosphorylation of STAT6 establishes a non-canonical type 2 immunity axis to prevent macrophage senescence. | ||
Nucleic Acids Res An RNAi screen of RNA helicases identifies eIF4A3 as a regulator of embryonic stem cell identity | ||
Cell Death Differ TRIM32 promotes anoikis resistance and metastasis in NSCLC by degrading CHEK2 to enhance IL-6 secretion |
Reviews
What customers say
All three reviews rate this antibody 5 stars for Western Blot at a 1:1000 dilution. Users report consistent, sharp bands and reliable performance across a range of samples, including HeLa cells, MDA-MB-231 cells, human keratinocytes, cardiomyocytes, and mouse liver and skin tissues. Overall, reviewers describe a positive experience with clear, specific results in their WB experiments.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Arnaud (Verified Customer) (08-04-2026) | Good antibody
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Md Asad (Verified Customer) (06-11-2026) | Good experience with sharp band in my western blots.
|
Priya (Verified Customer) (01-17-2023) | I have used this antibody for human keratinocytes, cardiomyocytes, mouse skin and liver tissues
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