Tested Applications
| Positive WB detected in | HeLa cells, THP-1 cells, U-251 cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14014-1-AP targets NFKBIZ in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5120 Product name: Recombinant human NFKBIZ protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 369-718 aa of BC060800 Sequence: AHLHSFSMMPSSACEAMVGHEMASDSSNTSLPFSNMGNPMNTTQLGKSLFQWQVEQEESKLANISQDQFLSKDADGDTFLHIAVAQGRRALSYVLARKMNALHMLDIKEHNGQSAFQVAVAANQHLIVQDLVNIGAQVNTTDCWGRTPLHVCAEKGHSQVLQAIQKGAVGSNQFVDLEATNYDGLTPLHCAVIAHNAVVHELQRNQQPHSPEVQELLLKNKSLVDTIKCLIQMGAAVEAKDRKSGRTALHLAAEEANLELIRLFLELPSCLSFVNAKAYNGNTALHVAASLQYRLTQLDAVRLLMRKGADPSTRNLENEQPVHLVPDGPVGEQIRRILKGKSIQQRAPPY Predict reactive species |
| Full Name | nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, zeta |
| Calculated Molecular Weight | 78 kDa |
| Observed Molecular Weight | 78 kDa, 64 kDa |
| GenBank Accession Number | BC060800 |
| Gene Symbol | NFKBIZ |
| Gene ID (NCBI) | 64332 |
| RRID | AB_2878000 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BYH8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NFKBIZ, also named as NF-kappa-B inhibitor zeta, is a 718 amino acid protein, which contains 7 ANK repeats and is expressed at high levels in peripheral blood leukocytes and lung, at moderate levels in liver, placenta, and at low levels in spleen, kidney, skeletal muscle and heart. NFKBIZ exists as three isoforms and molecular weight of isoform three is a 64 kDa. The isoform one is 78 kDa protein and isoform two is a 68 kDa protein. NFKBIZ is involved in regulation of NF-kappa-B transcription factor complexes. It Inhibits NF-kappa-B activity without affecting its nuclear translocation upon stimulation.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for NFKBIZ antibody 14014-1-AP | Download protocol |
| WB protocol for NFKBIZ antibody 14014-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NFKBIZ Polyclonal antibody (Cat# 14014-1-AP) has accumulated 15 citations published in journals including Advanced Materials, Cell Molecular Life Sciences, JCI Insight, Experimental Neurology, FASEB Journal, Phytomedicine, Frontiers in Immunology, and ACS Applied Bio Materials. Associated research fields encompass hepatocellular carcinoma, Alzheimer's disease, dry eye disease, cerebral ischemia/reperfusion, septic acute kidney injury, renal transplantation, glioblastoma, anxiety disorders, and hepatic oxidative damage.
| Species | Application | Title |
|---|---|---|
Exploration (Beijing) An Ocular Surface-Targeting Nucleic Acid Hydrogel for Efficient Dry Eye Disease Treatment. | ||
Adv Mater Membrane Fusion-Mediated Loading of Therapeutic siRNA into Exosome for Tissue-Specific Application | ||
J Nanobiotechnology Itaconate-functionalized tetrahedral framework nucleic acids break the dry eye vicious cycle through dual antioxidant and anti-inflammatory actions. | ||
Phytomedicine Ginkgo biloba Extract GBE50 ameliorates cerebrovascular dysfunction and cognitive impairment in a mouse model of Alzheimer's disease | ||
Int J Biol Macromol Charaterization and immunomodulatory activities of polysaccharide isolated from Pleurotus eryngii. | ||
Front Immunol Single-Cell Transcriptome Analysis of Chronic Antibody-Mediated Rejection After Renal Transplantation. |
