Tested Applications
| Positive WB detected in | A431 cells, HepG2 cells |
| Positive IHC detected in | rat salivary gland tissue, rat stomach tissue, mouse stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14062-1-AP targets PKP4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5113 Product name: Recombinant human PKP4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 800-1149 aa of BC050308 Sequence: DQWDGVGPIPGLSKSPKGVEMLWHPSVVKPYLTLLAESSNPATLEGSAGSLQNLSAGNWKFAAYIRAAVRKEKGLPILVELLRMDNDRVVSSVATALRNMALDVRNKELIGKYAMRDLVNRLPGGNGPSVLSDETMAAICCALHEVTSKNMENAKALADSGGIEKLVNITKGRGDRSSLKVVKAAAQVLNTLWQYRDLRSIYKKDGWNQNHFITPVSTLERDRFKSHPSLSTTNQQMSPIIQSGSSKPSPIYISSYSSPAREQNRRLQHQQLYYSQDDSNRKNFDAYRLYLQSPHSYEDPYFDDRVHFPASTDYSTQYGLKSTTNYVDFYSTKRPSYRAEQYPGSPDSWV Predict reactive species |
| Full Name | plakophilin 4 |
| Calculated Molecular Weight | 132 kDa |
| Observed Molecular Weight | 125-140 kDa |
| GenBank Accession Number | BC050308 |
| Gene Symbol | PKP4 |
| Gene ID (NCBI) | 8502 |
| RRID | AB_10640532 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99569 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PKP4 antibody 14062-1-AP | Download protocol |
| WB protocol for PKP4 antibody 14062-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PKP4 Polyclonal antibody (Cat# 14062-1-AP) has 1 total citation. It appears in Biochemical and Biophysical Research Communications (IF 2.5), in a study investigating how hydrogen sulfide improves keratinocyte migration and diabetic skin wound healing via plakophilin 4. The research field encompasses skin biology, wound healing, and diabetic complications. The antibody was used in Western Blot with human samples.
| Species | Application | Title |
|---|---|---|
Biochem Biophys Res Commun Hydrogen sulfide improves keratinocytes migration and diabetic skin wound healing via plakophilin 4.
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