MIPOL1 Polyclonal antibody

MIPOL1 Polyclonal Antibody for WB, ELISA

Cat No. 14158-1-AP

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

mirror image polydactyly 1, Mirror-image polydactyly gene 1 protein

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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WB analysis using 14158-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14158-1-AP (MIPOL1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in

HepG2 cells, HuH-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14158-1-AP targets MIPOL1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag5340 Product name: Recombinant human MIPOL1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-261 aa of BC035870 Sequence: MSRLQLAIEERDEAIARAKHMEMSLKVLENINPEENDMTLQELLNRINNADTGIAIQKNGAIIVDRIYKTKECKMRITAEEMSALIEERDAALSKCKRLEQELHHVKEQNQTSANNMRHLTAENNQERALKAKLLSMQQARETAVQQYKKLEEEIQTLRVYYSLHKSLSQEENLKDQFNYTLSTYEEALKNRENIVSITQQQNEELATQLQQALTERANMELQLQHAREASQVANEKVQKLERLVDVLRKKVGTGTMRTVI Predict reactive species
Full Name	mirror-image polydactyly 1
Calculated Molecular Weight	442 aa, 52 kDa
Observed Molecular Weight	52 kDa
GenBank Accession Number	BC035870
Gene Symbol	MIPOL1
Gene ID (NCBI)	145282
RRID	AB_2141930
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8TD10
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MIPOL1 (also named CCDC193) encodes a nuclear coiled-coil protein. Germline MIPOL1 variants cause Laurin‑Sandrow syndrome characterized by mirror-image polydactyly. It acts as a candidate tumor suppressor in nasopharyngeal carcinoma. Downregulation of MIPOL1 activates AKT/FAK signaling, elevates angiogenic cytokines, and promotes cancer invasion and metastasis. Restoration of wild-type MIPOL1 enhances RhoB activity and suppresses tumor progression both in vitro and in xenograft mouse models. (PMID: 31609475)