ITPKC Polyclonal antibody

ITPKC Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 14267-1-AP

Publications(1)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

IP3 3 kinase C, InsP 3-kinase C, InsP 3 kinase C, Inositol-trisphosphate 3-kinase C, Inositol 1,4,5-trisphosphate 3-kinase C

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

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Filter: AllWBIPIHCIF/ICC

WB analysis using 14267-1-AP
MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 14267-1-AP (ITPKC antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of human heart using 14267-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 14267-1-AP (ITPKC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of HepG2 using 14267-1-AP
IP result of anti-ITPKC (IP:14267-1-AP, 4ug; Detection:14267-1-AP 1:400) with HepG2 cells lysate 1600ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human breast cancer using 14267-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14267-1-AP (ITPKC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human breast cancer using 14267-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14267-1-AP (ITPKC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human lymphoma using 14267-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14267-1-AP (ITPKC Antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human lymphoma using 14267-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14267-1-AP (ITPKC Antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human heart using 14267-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14267-1-AP (ITPKC Antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human heart using 14267-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14267-1-AP (ITPKC Antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HepG2 using 14267-1-AP
Immunofluorescent analysis of HepG2 cells, using ITPKC antibody 14267-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	MDA-MB-453s cells, human heart tissue
Positive IP detected in	HepG2 cells
Positive IHC detected in	human breast cancer tissue, human heart tissue, human lymphoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14267-1-AP targets ITPKC in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag5583 Product name: Recombinant human ITPKC protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-358 aa of BC060788 Sequence: MRRCPCRGSLNEAEAGALPAAARMGLEAPRGGRRRQPGQQRPGPGAGAPAGRPEGGGPWARTEGSSLHSEPERAGLGPAPGTESPQAEFWTDGQTEPAAAGLGVETERPKQKTEPDRSSLRTHLEWSWSELETTCLWTETGTDGLWTDPHRSDLQFQPEEASPWTQPGVHGPWTELETHGSQTQPERVKSWADNLWTHQNSSSLQTHPEGACPSKEPSADGSWKELYTDGSRTQQDIEGPWTEPYTDGSQKKQDTEAARKQPGTGGFQIQQDTDGSWTQPSTDGSQTAPGTDCLLGEPEDGPLEEPEPGELLTHLYSHLKCSPLCPVPRLIITPETPEPEAQPVGPPSRVEGGSGGFS Predict reactive species
Full Name	inositol 1,4,5-trisphosphate 3-kinase C
Calculated Molecular Weight	75 kDa
Observed Molecular Weight	100-105 kDa
GenBank Accession Number	BC060788
Gene Symbol	ITPKC
Gene ID (NCBI)	80271
RRID	AB_2280728
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96DU7
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ITPKC(Inositol-trisphosphate 3-kinase C) is also named as IP3KC and belongs to the inositol phosphokinase (IPK) family. It can phosphorylate inositol 2,4,5-triphosphate to inositol 2,4,5,6-tetraphosphate and is relatively weakly activated by the calcium-calmodulin complex. ITPKC also acts as a negative regulator of T-cell activation through the Ca(2+)/NFAT signaling pathway, and the C allele may contribute to immune hyperreactivity in Kawasaki disease(PMID:17160344).

Protocols

Product Specific Protocols
IF protocol for ITPKC antibody 14267-1-AP	Download protocol
IHC protocol for ITPKC antibody 14267-1-AP	Download protocol
IP protocol for ITPKC antibody 14267-1-AP	Download protocol
WB protocol for ITPKC antibody 14267-1-AP	Download protocol