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NIT1 Polyclonal antibody

NIT1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 14380-1-AP
Publications (1)
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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Deaminated glutathione amidase, dGSH amidase, EC:3.5.1.128, nitrilase 1, Nitrilase homolog 1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
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  • Western Blot (WB) analysis of various lysates using NIT1 Polyclonal antibody (14380-1-AP)

    WB analysis using 14380-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14380-1-AP (NIT1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Western Blot (WB) analysis of HeLa cells using NIT1 Polyclonal antibody (14380-1-AP)

    WB analysis of HeLa using 14380-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14380-1-AP (NIT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Western Blot (WB) analysis of HEK-293T cells using NIT1 Polyclonal antibody (14380-1-AP)

    WB analysis of HEK-293T using 14380-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 14380-1-AP (NIT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using NIT1 Polyclonal antibody (14380-1-AP)

    IHC staining of human pancreas cancer using 14380-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14380-1-AP (NIT1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using NIT1 Polyclonal antibody (14380-1-AP)

    IHC staining of human pancreas cancer using 14380-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14380-1-AP (NIT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using NIT1 Polyclonal antibody (14380-1-AP)

    IHC staining of human pancreas cancer using 14380-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14380-1-AP (NIT1 antibody) at dilution of 1:50 (under 10x lens).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using NIT1 Polyclonal antibody (14380-1-AP)

    IHC staining of human pancreas cancer using 14380-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14380-1-AP (NIT1 antibody) at dilution of 1:50 (under 40x lens).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NIT1 Polyclonal antibody (14380-1-AP)

    IF Staining of HepG2 using 14380-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 14380-1-AP (NIT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, HEK-293T cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14380-1-AP targets NIT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5764

Product name: Recombinant human NIT1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-243 aa of BC046149

Sequence: MLGFITRPPHRFLSLLCPGLRIPQLSVLCAQPRPRAMAISSSSCELPLVAVCQVTSTPDKQQNFKTCAELVREAARLGPCLAFLPEAFDFIARDPAETLHLSEPLGGKLLEEYTQLARECGLWLSLGGFHERGQDWEQTQKIYNCHVLLNSKGAVVATYRKTHLCDVEIPGQGPMCESNSTMPGPSLESPVSTPAGKIGLAVCYDMRFPELSLALAQAGAEILTYPSAFGSITGPAHWEPVSS

Predict reactive species
Full Name nitrilase 1
Calculated Molecular Weight 40 kDa
Observed Molecular Weight 32-36 kDa
GenBank Accession NumberBC046149
Gene Symbol NIT1
Gene ID (NCBI) 4817
RRIDAB_2151314
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86X76
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for NIT1 antibody 14380-1-APDownload protocol
IHC protocol for NIT1 antibody 14380-1-APDownload protocol
WB protocol for NIT1 antibody 14380-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The NIT1 Polyclonal antibody (Cat# 14380-1-AP) has 1 citation. It appears in Cell Death & Disease (2018), a study investigating how NIT1 suppresses tumour proliferation by activating the TGFβ1-Smad2/3 signalling pathway in colorectal cancer. The research fields associated with this citation include colorectal cancer biology, TGFβ signalling pathway, and tumour cell proliferation. The antibody was used for WB and IHC applications in human samples.

Automatically generated from the 1 publication citations by Able AI.
SpeciesApplicationTitle
humanWB,IHC

Cell Death Dis

NIT1 suppresses tumour proliferation by activating the TGFβ1-Smad2/3 signalling pathway in colorectal cancer.

Authors - Chun Lin
  • KD Validated
View Article
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