Tested Applications
| Positive WB detected in | CTLL-2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14580-1-AP targets Perforin in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6060 Product name: Recombinant human Perforin protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 247-555 aa of BC063043 Sequence: EGLTDNEVEDCLTVEAQVNIGIHGSISAEAKACEEKKKKHKMTASFHQTYRERHSEVVGGHHTSINDLLFGIQAGPEQYSAWVNSLPGSPGLVDYTLEPLHVLLDSQDPRREALRRALSQYLTDRARWRDCSRPCPPGRQKSPRDPCQCVCHGSAVTTQDCCPRQRGLAQLEVTFIQAWGLWGDWFTATDAYVKLFFGGQELRTSTVWDNNNPIWSVRLDFGDVLLATGGPLRLQVWDQDSGRDDDLLGTCDQAPKSGSHEVRCNLNHGHLKFRYHARCLPHLGGGTCLDYVPQMLLGEPPGNRSGAVW Predict reactive species |
| Full Name | perforin 1 (pore forming protein) |
| Calculated Molecular Weight | 61 kDa |
| Observed Molecular Weight | 70-75 kDa |
| GenBank Accession Number | BC063043 |
| Gene Symbol | Perforin |
| Gene ID (NCBI) | 5551 |
| RRID | AB_10639524 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P14222 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Perforin 1 (PRF1) is one of the major cytolytic proteins of cytolytic granules. It is known to be a crucial effector molecule in cytolytic T lymphocyte and natural killer cell-mediated cytotoxicity. This protein has structural and functional similarities to complement component C9. Like C9, this protein creates transmembrane tubules and is capable of lysing nonspecifically a variety of target cells. Defects in PRF1 are the cause of familial hemophagocytic lymphohistiocytosis type 2 (FHL2), which is characterized by immune dysregulation with hypercytokinemia and defective natural killer cell function. (PMID: 2417226; 7774276; 2783486; 10583959)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Perforin antibody 14580-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Perforin polyclonal antibody (Cat# 14580-1-AP) has accumulated 26 citations published in journals including Cancer Cell, Cell Metabolism, Neuron, Cell Reports, iScience, Frontiers in Immunology, Molecular Immunology, European Journal of Pharmacology, International Immunopharmacology, Biochimica et Biophysica Acta, and Developmental and Comparative Immunology. Associated research fields encompass cancer immunotherapy, NK cell cytotoxicity, T-cell immune responses, immune checkpoint blockade, tumor microenvironment, liver fibrosis, and comparative immunology.
| Species | Application | Title |
|---|---|---|
Cancer Cell B cells disrupt tertiary lymphoid structure formation and suppress anti-tumor immunity. | ||
Cell Metab HEBP2-governed glutamine competition between tumor and macrophages dictates immunotherapy efficacy in triple-negative breast cancer | ||
Neuron Metabolic reprogramming through histone lactylation in microglia and macrophages recruits CD8+ T lymphocytes and aggravates spinal cord injury | ||
J Exp Clin Cancer Res Targeting MUS81 promotes the anticancer effect of WEE1 inhibitor and immune checkpoint blocking combination therapy via activating cGAS/STING signaling in gastric cancer cells. | ||
Cell Rep Med Circulating natural killer cells are phenotypically and functionally altered in age-related macular degeneration. | ||
Carbohydr Polym Structural characterization and antitumor activity via immune modulation of an α-glucan from Grifola frondosa. |
