Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells, K-562 cells, NIH/3T3 cells, mouse brain, rat brain |
| Positive IHC detected in | human colon cancer tissue, mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14652-1-AP targets ARPC3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6249 Product name: Recombinant human ARPC3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-178 aa of BC067747 Sequence: MPAYHSSLMDPDTKLIGNMALLPIRSQFKGPAPRETKDTDIVDEAIYYFKANVFFKNYEIKNEADRTLIYITLYISECLKKLQKCNSKSQGEKEMYTLGITNFPIPGEPGFPLNAIYAKPANKQEDEVMRAYLQQLRQETGLRLCEKVFDPQNDKPSKWWTCFVKRQFMNKSLSGPGQ Predict reactive species |
| Full Name | actin related protein 2/3 complex, subunit 3, 21kDa |
| Calculated Molecular Weight | 21 kDa |
| Observed Molecular Weight | 21 kDa |
| GenBank Accession Number | BC067747 |
| Gene Symbol | ARPC3 |
| Gene ID (NCBI) | 10094 |
| RRID | AB_2059933 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15145 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ARPC3 antibody 14652-1-AP | Download protocol |
| WB protocol for ARPC3 antibody 14652-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ARPC3 polyclonal antibody (Cat# 14652-1-AP) has 10 citations spanning journals including Nature Immunology, Nature Structural & Molecular Biology, Cell Reports, Journal of Cell Biology, EMBO Reports, Biomolecules, Journal of Ethnopharmacology, International Journal of Molecular Sciences, and Nature Communications. Research fields cover hemolysis-induced infection, cancer metastasis, angiogenesis, actin polymerization, HSP70-mediated apoptosis, mastitis inflammation, and melanosome maturation.
| Species | Application | Title |
|---|---|---|
Nat Immunol Heme drives hemolysis-induced susceptibility to infection via disruption of phagocyte functions. | ||
Cell Rep Nuclear translocation of cGAS orchestrates VEGF-A-mediated angiogenesis
| ||
J Ethnopharmacol Compound kushen injection inhibits breast cancer lung metastasis through regulating MTSS1/ARPC3/F-actin | ||
J Cell Biol α5β1 integrin recycling promotes Arp2/3-independent cancer cell invasion via the formin FHOD3.
| ||
EMBO Rep WDR63 inhibits Arp2/3-dependent actin polymerization and mediates the function of p53 in suppressing metastasis. |
Reviews
What customers say
The single review rates this antibody 5 stars for Western Blot on neonatal microglia. The user reports excellent performance at a 1:1000 dilution with 5% milk in TBS-T blocking, yielding one specific, clear band. Overall, the reviewer describes it as an awesome antibody that works great for WB.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Kiara (Verified Customer) (11-15-2021) | Works great for Western Blot! We used 5% milk in TBS-T to block the membrane and diluted the ARPC3 antibody 1:1000 in 5% milk in TBS-T. We received one specific clear band. Awesome antibody!
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