Tested Applications
| Positive WB detected in | HEK-293 cells, Jurkat cells, Y79 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14735-1-AP targets Muskelin in WB, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6147 Product name: Recombinant human MKLN1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 388-735 aa of BC067825 Sequence: FDHQMCMDSEKHMIYTFGGRILTCNGSVDDSRASEPQFSGLFAFNCQCQTWKLLREDSCNAGPEDIQSRIGHCMLFHSKNRCLYVFGGQRSKTYLNDFFSYDVDSDHVDIISDGTKKDSGMVPMTGFTQRATIDPELNEIHVLSGLSKDKEKREENVRNSFWIYDIVRNSWSCVYKNDQAAKDNPTKSLQEEEPCPRFAHQLVYDELHKVHYLFGGNPGKSCSPKMRLDDFWSLKLCRPSKDYLLRHCKYLIRKHRFEEKAQVDPLSALKYLQNDLYITVDHSDPEETKEFQLLASALFKSGSDFTALGFSDVDHTYAQRTQLFDTLVNFFPDSMTPPKGNLVDLITL Predict reactive species |
| Full Name | muskelin 1, intracellular mediator containing kelch motifs |
| Calculated Molecular Weight | 735 aa, 85 kDa |
| Observed Molecular Weight | 70-75 kDa |
| GenBank Accession Number | BC067825 |
| Gene Symbol | Muskelin |
| Gene ID (NCBI) | 4289 |
| RRID | AB_2142504 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UL63 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Muskelin antibody 14735-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
| Species | Application | Title |
|---|---|---|
Neuron Muskelin Coordinates PrPC Lysosome versus Exosome Targeting and Impacts Prion Disease Progression.
| ||
Sci Rep Tagging enhances histochemical and biochemical detection of Ran Binding Protein 9 in vivo and reveals its interaction with Nucleolin. | ||
Mol Cell mTORC1-CTLH E3 ligase regulates the degradation of HMG-CoA synthase 1 through the Pro/N-degron pathway
| ||
Cell Rep mTORC1 regulates the pyrimidine salvage pathway by controlling UCK2 turnover via the CTLH-WDR26 E3 ligase |
Reviews
What customers say
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
S (Verified Customer) (12-31-2021) | This antibody is ok, but not the best. At least, KO validation. The weak signal could be due to the low protein expression level though.
 |
H (Verified Customer) (04-05-2020) | Primary antibody: 1:1000 dilution in 5% BSA, over night at 4CSecondary antibody: Rabbit IRDye800Detection: odyssey ClxI was able to detect the band in HEK293T and HCT116 cells. However, the band intensity was not that strong.
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