Tested Applications
| Positive WB detected in | mouse heart tissue, pig kidney tissue, mouse kidney tissue, rat heart tissue, rat kidney tissue |
| Positive IHC detected in | human kidney tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14937-1-AP targets URAT1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6770 Product name: Recombinant human URAT1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 4-351 aa of BC053348 Sequence: SELLDLVGGLGRFQVLQTMALMVSIMWLCTQSMLENFSAAVPSHRCWAPLLDNSTAQASILGSLSPEALLAISIPPGPNQRPHQCRRFRQPQWQLLDPNATATSWSEADTEPCVDGWVYDRSIFTSTIVAKWNLVCDSHALKPMAQSIYLAGILVGAAACGPASDRFGRRLVLTWSYLQMAVMGTAAAFAPAFPVYCLFRFLLAFAVAGVMMNTGTLLMEWTAARARPLVMTLNSLGFSFGHGLTAAVAYGVRDWTLLQLVVSVPFFLCFLYSWWLAESARWLLTTGRLDWGLQELWRVAAINGKGAVQDTLTPEVLLSAMREELSMGQPPASLGTLLRMPGLRFRTC Predict reactive species |
| Full Name | solute carrier family 22 (organic anion/urate transporter), member 12 |
| Calculated Molecular Weight | 60 kDa |
| Observed Molecular Weight | 65-70 kDa, 40 kDa |
| GenBank Accession Number | BC053348 |
| Gene Symbol | URAT1 |
| Gene ID (NCBI) | 116085 |
| RRID | AB_2191270 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96S37 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Human urate anion exchanger (URAT1), also known as SLC22A12, is a urate transporter located in the proximal tubules of human kidney and responsible for urate reabsorption. Defects in gene encoding URAT1 cause hypouricemia renal type 1 (RHUC1). This antibody recognizes the native and glycosylated URAT1 between 65 and 70 kDa as well as the truncated 40kDa protein.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for URAT1 antibody 14937-1-AP | Download protocol |
| IHC protocol for URAT1 antibody 14937-1-AP | Download protocol |
| WB protocol for URAT1 antibody 14937-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The URAT1 Polyclonal antibody (Cat# 14937-1-AP) has 127 citations. These appear across journals such as Phytomedicine, Journal of Ethnopharmacology, Journal of Agricultural and Food Chemistry, Food & Function, Frontiers in Pharmacology, Nutrients, and International Journal of Biological Macromolecules. Research fields center on hyperuricemia and gout, uric acid metabolism and urate transporters, hyperuricemic nephropathy/renal injury, gut microbiota modulation, and related signaling pathways (NLRP3, PI3K/AKT, NF-κB). Most studies use WB, IF, and IHC in mouse, rat, and human models.
| Species | Application | Title |
|---|---|---|
J Adv Res Punicalagin attenuates hyperuricemia via restoring hyperuricemia-induced renal and intestinal dysfunctions | ||
J Neuroinflammation Urate inhibits microglia activation to protect neurons in an LPS-induced model of Parkinson's disease. | ||
Phytomedicine Identifying the natural compounds in Jinqian Xuduan Decoction for simultaneous uric acid lowering and chronic kidney disease amelioration | ||
Phytomedicine Oxyberberrubine, a novel liver microsomes-mediated secondary metabolite of berberine, alleviates hyperuricemic nephropathy in mice | ||
Phytomedicine Amelioration effects of α-viniferin on hyperuricemia and hyperuricemia-induced kidney injury in mice | ||
Phytomedicine Antihyperuricemia and antigouty arthritis effects of Persicaria capitata herba in mice. |
