Tested Applications
| Positive WB detected in | mouse brain tissue, human placenta tissue, human testis tissue, mouse bladder tissue, human heart tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human kidney tissue, human liver tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14944-1-AP targets GGPS1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6745 Product name: Recombinant human GGPS1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-300 aa of BC067768 Sequence: MEKTQETVQRILLEPYKYLLQLPGKQVRTKLSQAFNHWLKVPEDKLQIIIEVTEMLHNASLLIDDIEDNSKLRRGFPVAHSIYGIPSVINSANYVYFLGLEKVLTLDHQDAVKLFTRQLLELHQGQGLDIYWRDNYTCPTEEEYKAMVLQKTGGLFGLAVGLMQLFSDYKEDLKPLLNTLGLFFQIRDDYANLHSKEYSENKSFCEDLTEGKFSFPTIHAIWSRPESTQVQNILRQRTENIDIKKYCVHYLEDVGSFEYTRNTLKELEAKAYKQIDARGGNPELVALVKHLSKMFKEENE Predict reactive species |
| Full Name | geranylgeranyl diphosphate synthase 1 |
| Calculated Molecular Weight | 35 kDa |
| Observed Molecular Weight | 35 kDa |
| GenBank Accession Number | BC067768 |
| Gene Symbol | GGPS1 |
| Gene ID (NCBI) | 9453 |
| RRID | AB_2110990 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95749 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
GGPS1(Geranylgeranyl pyrophosphate synthase) belongs to the FPP/GGPP synthase family and catalyzes the synthesis of GGPP, an important molecule responsible for the C20-prenylation of protein and for the regulation of a nuclear hormone receptor. GGPP synthase puri¢ed from bovine brain is reported to be a homo-oligomer (150-195 kDa) with a molecular mass of 37.5 kDa for the monomer. This is consistent with the calculated molecular mass (35 kDa) of mouse and human GGPP synthases (PMID:10101267).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for GGPS1 antibody 14944-1-AP | Download protocol |
| IP protocol for GGPS1 antibody 14944-1-AP | Download protocol |
| WB protocol for GGPS1 antibody 14944-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GGPS1 Polyclonal antibody (Cat# 14944-1-AP) has 17 citations spanning journals including Nature Cancer, Developmental Cell, Cell Reports, FASEB Journal, FEBS Journal, International Journal of Cancer, BMC Cancer, American Journal of Pathology, and Oncology Letters. Research fields cover oncology (lung and liver cancer), immunology and inflammation, developmental biology, lipid metabolism, kidney disease, atherosclerosis, and ferroptosis, with cited applications in WB, IHC, IF, and FC.
| Species | Application | Title |
|---|---|---|
Nat Cancer Therapeutic targeting of the mevalonate-geranylgeranyl diphosphate pathway with statins overcomes chemotherapy resistance in small cell lung cancer.
| ||
Dev Cell Quantitative proteomic profiling identifies global protein network dynamics in murine embryonic heart development | ||
Int J Biol Macromol Targeting geranylgeranyl diphosphate synthase suppresses interleukin-1β-driven proliferation in lung squamous cell carcinoma by inhibiting Ras homolog family member A and Rac family small GTPase 1 geranylgeranylation. | ||
Cell Rep ENKD1 modulates innate immune responses through enhanced geranylgeranyl pyrophosphate synthase activity | ||
Cells Cysteine-Rich LIM-Only Protein 4 (CRP4) Promotes Atherogenesis in the ApoE-/- Mouse Model. | ||
Int Immunopharmacol Geranylgeranyl diphosphate synthase 1 knockdown suppresses NLRP3 inflammasome activity via promoting autophagy in sepsis-induced acute lung injury. |
Reviews
What customers say
All four reviews are for Western Blot at a 1:1000 dilution. Users consistently report the antibody works well, describing it as reliable, trustworthy, and dependable for reproducible results. One reviewer noted it performed well in uveal melanoma cell lines, though some background signal was observed, and the target was expressed in certain cell lines but not others. Overall, the antibody receives high ratings (4–5 stars) and is regarded as a solid, consistent choice for detecting its target in cancer cell applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Kristen (Verified Customer) (03-30-2026) | Used to investigate expression of GGPS-1 in various uveal melanoma cell lines. Worked pretty well although a little bit of background signal observed. Expressed in some uveal cell lines but not in others
|
Casey (Verified Customer) (12-02-2025) | Antibody worked well
|
Haley (Verified Customer) (11-25-2025) | Trustworthy for reproducible results
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Haley (Verified Customer) (11-25-2025) | Reliable and dependable antibody
|
