Tested Applications
| Positive WB detected in | Caco-2 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse kidney tissue |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15003-1-AP targets RENALASE in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13061 Product name: Recombinant human RENALASE protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-342 aa of BC005364 Sequence: MAQVLIVGAGMTGSLCAALLRRQTSGPLYLAVWDKADDSGGRMTTACSPHNPQCTADLGAQYITCTPHYAKKHQRFYDELLAYGVLRPLSSPIEGMVMKEGDCNFVAPQGISSIIKHYLKESGAEVYFRHRVTQINLRDDKWEVSKQTGSPEQFDLIVLTMPVPEILQLQGDITTLISECQRQQLEAVSYSSRYALGLFYEAGTKIDVPWAGQYITSNPCIRFVSIDNKKRNIESSEIGPSLVIHTTVPFGVTYLEHSIEDVQELVFQQLENILPGLPQPIATKCQKWRHSQVTNAAANCPGQMTLHHKPFLACGGDGFTQSNFDGCITSALCVLEALKNYI Predict reactive species |
| Full Name | chromosome 10 open reading frame 59 |
| Calculated Molecular Weight | 38 kDa |
| Observed Molecular Weight | 35 kDa |
| GenBank Accession Number | BC005364 |
| Gene Symbol | RENALASE |
| Gene ID (NCBI) | 55328 |
| RRID | AB_1557298 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5VYX0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RNLS, also named as Renalase, C10orf59 and MAO-C, belongs to the renalase family. It is probable FAD-dependent amine oxidase secreted by the kidney, which circulates in blood and modulates cardiac function and systemic blood pressure. RNLS degrades catecholamines such as dopamine, norepinephrine and epinephrine in vitro. It lowers blood pressure in vivo by decreasing cardiac contractility and heart rate and preventing a compensatory increase in peripheral vascular tone, suggesting a causal link to the increased plasma catecholamine and heightened cardiovascular risk. High concentrations of catecholamines activate plasma renalase and promotes its secretion and synthesis. RNLS has physiologically relevant catecholamine-oxidizing activity. (PMID:15841207 ) This antibody is specific to RNLS.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RENALASE antibody 15003-1-AP | Download protocol |
| IHC protocol for RENALASE antibody 15003-1-AP | Download protocol |
| IP protocol for RENALASE antibody 15003-1-AP | Download protocol |
| WB protocol for RENALASE antibody 15003-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-Renalase antibody (Cat# 15003-1-AP) has 10 total citations. These appear in journals including Circulation, Kidney International, Journal of Clinical Investigation, Life Sciences, Journal of Hypertension, Journal of Enzyme Inhibition and Medicinal Chemistry, Experimental Biology and Medicine, Frontiers in Oncology, Frontiers in Cardiovascular Medicine, and NDT Plus. Research fields span renal/cardiovascular biology, myocardial fibrosis, oxidative stress, hypertension, kidney transplantation, aldosterone regulation, and ovarian cancer/ferroptosis.
| Species | Application | Title |
|---|---|---|
J Clin Invest Renalase is a novel, soluble monoamine oxidase that regulates cardiac function and blood pressure. | ||
Life Sci Renalase is localized to the small intestine crypt and expressed upon the activation of NF-κB p65 in mice model of fasting-induced oxidative stress. | ||
J Hypertens Renalase mediates macrophage-to-fibroblast crosstalk to attenuate pressure overload-induced pathological myocardial fibrosis | ||
J Enzyme Inhib Med Chem Renalase stimulates aldosterone production via PMCA4b/cAMP in NCI-H295R cells. |
