Tested Applications
| Positive WB detected in | HEK-293 cells, HL-60 cells, mouse brain tissue, mouse kidney tissue |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15006-1-AP targets ARIH2 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6507 Product name: Recombinant human ARIH2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 140-493 aa of BC000422 Sequence: AVCMQFVRKENLLSLACQHQFCRSCWEQHCSVLVKDGVGVGVSCMAQDCPLRTPEDFVFPLLPNEELREKYRRYLFRDYVESHYQLQLCPGADCPMVIRVQEPRARRVQCNRCNEVFCFKCRQMYHAPTDCATIRKWLTKCADDSETANYISAHTKDCPKCNICIEKNGGCNHMQCSKCKHDFCWMCLGDWKTHGSEYYECSRYKENPDIVNQSQQAQAREALKKYLFYFERWENHNKSLQLEAQTYQRIHEKIQERVMNNLGTWIDWQYLQNAAKLLAKCRYTLQYTYPYAYYMESGPRKKLFEYQQAQLEAEIENLSWKVERADSYDRGDLENQMHIAEQRRRTLLKDFHDT Predict reactive species |
| Full Name | ariadne homolog 2 (Drosophila) |
| Calculated Molecular Weight | 58 kDa |
| Observed Molecular Weight | 59 kDa |
| GenBank Accession Number | BC000422 |
| Gene Symbol | ARIH2 |
| Gene ID (NCBI) | 10425 |
| RRID | AB_2290024 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95376 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ARIH2(Protein ariadne-2 homolog) is also named as ARI2, TRIAD1 and belongs to the RBR family and ariadne subfamily .It mediates 'Lys-48'-and 'Lys-63'-linked polyubiquitination and subsequent proteasomal degradation of modified proteins.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ARIH2 antibody 15006-1-AP | Download protocol |
| IP protocol for ARIH2 antibody 15006-1-AP | Download protocol |
| WB protocol for ARIH2 antibody 15006-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ARIH2 Polyclonal antibody (Cat# 15006-1-AP) has 9 citations spanning journals including Cancer Cell, Nature Immunology, Cell Death & Disease, Oncogene, International Journal of Surgery, EMBO Journal, Free Radical Biology and Medicine, and Journal of Biological Chemistry. Research fields cover CAR-NK cell therapy, embryogenesis and hematopoiesis, gastric cancer, acute myeloid leukemia, cholangiocarcinoma, pancreatic cancer, brown adipose tissue development, ferroptosis, and myeloid leukemogenesis.
| Species | Application | Title |
|---|---|---|
Cancer Cell Genome-wide CRISPR screens identify critical targets to enhance CAR-NK cell antitumor potency | ||
Nat Immunol ARIH2 is essential for embryogenesis, and its hematopoietic deficiency causes lethal activation of the immune system. | ||
Cell Death Dis ARIH2 regulates the proliferation, DNA damage and chemosensitivity of gastric cancer cells by reducing the stability of p21 via ubiquitination.
| ||
Oncogene PPDPF-mediated regulation of BCAA metabolism enhances mTORC1 activity and drives cholangiocarcinoma progression | ||
Oncogene The E3 ubiquitin ligase Triad1 influences development of Mll-Ell-induced acute myeloid leukemia. | ||
Int J Surg LINC00476 cooperates with ARIH2 and suppresses pancreatic cancer progression by inducing VIM ubiquitination.
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Reviews
What customers say
The single review for this antibody rates it 4 out of 5 stars. The reviewer used it for Western Blot at a 1:2000 dilution on mouse brain lysate and found it reliable for detecting endogenous ARIH2, noting that the detected band appeared at the correct molecular weight, slightly smaller than ARIH1. Overall, the feedback is positive, highlighting the antibody's specificity and appropriate molecular weight recognition in a standard WB application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Nou (Verified Customer) (01-02-2026) | A reliable Ab to detect endo ARIH2 with a right MW slightly smaller than ARIH1.
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