Tested Applications
| Positive WB detected in | LNCaP cells |
| Positive IHC detected in | mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15061-1-AP targets DHRS7 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7064 Product name: Recombinant human DHRS7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 27-183 aa of BC000637 Sequence: RADGDLTLLWAEWQGRRPEWELTDMVVWVTGASSGIGEELAYQLSKLGVSLVLSARRVHELERVKRRCLENGNLKEKDILVLPLDLTDTGSHEAATKAVLQEFGRIDILVNNGGMSQRSLCMDTSLDVYRKLIELNYLGTVSLTKCVLPHMIERKQG Predict reactive species |
| Full Name | dehydrogenase/reductase (SDR family) member 7 |
| Calculated Molecular Weight | 38 kDa |
| Observed Molecular Weight | 32-38 kDa |
| GenBank Accession Number | BC000637 |
| Gene Symbol | DHRS7 |
| Gene ID (NCBI) | 51635 |
| RRID | AB_3741858 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y394 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DHRS7 (Dehydrogenase/Reductase (SDR family) member 7), also known as SDR34C1, retSDR4, is a member of a large family of short-chain dehydrogenases/reductases (SDR). The DHRS7 gene codes for two subtypes and is found on chromosome 14. Isotype 1 (38 kDa) has 339 amino acids, while isotype 2 (32 kDa) contains 289 amino acids (PMID: 26466768). DHRS7 has been demonstrated to be an integral protein facing the lumen of the endoplasmic reticulum with lack of posttranscriptional glycosylation modification(PMID: 24246760).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for DHRS7 antibody 15061-1-AP | Download protocol |
| IHC protocol for DHRS7 antibody 15061-1-AP | Download protocol |
| WB protocol for DHRS7 antibody 15061-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The DHRS7 Polyclonal antibody (Cat# 15061-1-AP) has 1 total citation. The cited publication appeared in Frontiers in Immunology (Impact Factor: 7), published in January 2026. The research focuses on immune cell interaction aberrations in necrotizing enterocolitis and the regulatory role of DHRS7, placing it within the fields of immunology and pediatric gastrointestinal disease. The application used was IHC in human samples.
| Species | Application | Title |
|---|---|---|
Front Immunol Deciphering immune cell interaction aberrations in necrotizing enterocolitis: the regulatory role of DHRS7. |
