Tested Applications
| Positive WB detected in | PC-3 cells, HeLa cells, SH-SY5Y cells |
| Positive IHC detected in | mouse brain tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | hTERT-RPE1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15072-1-AP targets TTBK2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7108 Product name: Recombinant human TTBK2 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 1-354 aa of BC071556 Sequence: MSGGGEQPDILSVGILVKERWKVLRKIGGGGFGEIYDALDMLTRENVALKVESAQQPKQVLKMEVAVLKKLQGKDHVCRFIGCGRNDRFNYVVMQLQGRNLADLRRSQSRGTFTISTTLRLGRQILESIESIHSVGFLHRDIKPSNFAMGRFPSTCRKCYMLDFGLARQFTNSCGDVRPPRAVAGFRGTVRYASINAHRNREMGRHDDLWSLFYMLVEFVVGQLPWRKIKDKEQVGSIKERYDHRLMLKHLPPEFSIFLDHISSLDYFTKPDYQLLTSVFDNSIKTFGVIESDPFDWEKTGNDGSLTTTTTSTTPQLHTRLTPAAIGIANATPIPGDLLRENTDEVFPDEQLSD Predict reactive species |
| Full Name | tau tubulin kinase 2 |
| Calculated Molecular Weight | 137 kDa |
| Observed Molecular Weight | 137-150 kDa |
| GenBank Accession Number | BC071556 |
| Gene Symbol | TTBK2 |
| Gene ID (NCBI) | 146057 |
| RRID | AB_2211507 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6IQ55 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TTBK2(tau tubulin kinase 2) plays an important role in the tau cascade and in spinocerebellar degeneration. The N-terminus of TTBK2 has a serine-threonine-tyrosine kinase domain, and a C-terminal region shows homology to TTBK1. TTBK1 and TTBK2 function as regulators of TDP-43 phosphorylation. TTBK1/2 may be attractive drug targets for therapeutic interventions in TDP-43 proteinopathies such as FTLD-TDP and ALS.(PMID:25473830). 15072-1-AP has a cross reaction with TTBK1.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TTBK2 antibody 15072-1-AP | Download protocol |
| IHC protocol for TTBK2 antibody 15072-1-AP | Download protocol |
| WB protocol for TTBK2 antibody 15072-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TTBK2 Polyclonal antibody (Cat# 15072-1-AP) has 12 citations published in journals including Cell Research, JCI, EMBO Reports, PLoS Genetics, J Cell Science, Human Molecular Genetics, eLife, Scientific Reports, Cell Communication and Signaling, Neuroscience Bulletin, and bioRxiv. Research fields span ciliogenesis, autophagy, TDP-43 proteinopathy, liver fibrosis, spinocerebellar ataxia, schizophrenia, nucleotide excision repair, and cell invasion.
| Species | Application | Title |
|---|---|---|
Cell Res NudCL2 is an autophagy receptor that mediates selective autophagic degradation of CP110 at mother centrioles to promote ciliogenesis. | ||
J Clin Invest TGF-β signaling promotes astroglial activation and TDP-43 proteinopathy in organoid models of frontotemporal lobar degeneration. | ||
Cell Commun Signal Checkpoint kinase 2 coordinates autophagy activation and Aurora kinase A degradation to regulate primary cilia for cell invasion. | ||
Neurosci Bull Downregulation of UQCRC1 Expression Leads to Cognitive Decline by Disrupting the Cilia in the Dentate Gyrus. | ||
EMBO Rep XIAP-mediated degradation of IFT88 disrupts HSC cilia to stimulate HSC activation and liver fibrosis | ||
Sci Rep MCRS1 associates with cytoplasmic dynein and mediates pericentrosomal material recruitment. |
Reviews
What customers say
The single review reports using this antibody at 1:1000 dilution on HEK293T cell lysates with a few hours of room-temperature incubation. It detected two main bands: one above the 118 kDa marker (consistent with the expected TTBK2 size of 137–150 kDa) and another between the 47 and 85 kDa markers. The reviewer rated it 3 out of 5, suggesting the antibody works for Western Blot but may show some non-specific or additional bands.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Christine (Verified Customer) (01-27-2023) | Used on lysates from HEK293T cells. Incubation for a few hours at room temperature. Detection of 2 main bands, 1 above 118 kDa marker (expected size of TTBK2 is 137-150 kDa) and 1 between 47 and 85 kDa markers.
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