Tested Applications
| Positive WB detected in | HepG2 cells, mouse pancreas tissue, PC-3 cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:300-1:600 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15078-1-AP targets PFDN5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7150 Product name: Recombinant human PFDN5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-154 aa of BC003373 Sequence: MAQSINITELNLPQLEMLKNQLDQEVEFLSTSIAQLKVVQTKYVEAKDCLNVLNKSNEGKELLVPLTSSMYVPGKLHDVEHVLIDVGTGYYVEKTAEDAKDFFKRKIDFLTKQMEKIQPALQEKHAMKQAVMEMMSQKIQQLTALGAAQATAKA Predict reactive species |
| Full Name | prefoldin subunit 5 |
| Calculated Molecular Weight | 17 kDa |
| Observed Molecular Weight | 17 kDa |
| GenBank Accession Number | BC003373 |
| Gene Symbol | PFDN5 |
| Gene ID (NCBI) | 5204 |
| RRID | AB_2162703 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99471 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PFDN5 antibody 15078-1-AP | Download protocol |
| IHC protocol for PFDN5 antibody 15078-1-AP | Download protocol |
| WB protocol for PFDN5 antibody 15078-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PFDN5 Polyclonal antibody (Cat# 15078-1-AP) has 1 citation. It appears in Brain Sciences (2022, IF 3.4), in a study on identifying novel pathogenesis biomarkers to predict neurological outcomes after cardiac arrest. The research field is neuroscience/cardiology, with the antibody used in Western blot on rat samples.
| Species | Application | Title |
|---|---|---|
Brain Sci Identification and Validation of Novel Potential Pathogenesis and Biomarkers to Predict the Neurological Outcome after Cardiac Arrest |
Reviews
What customers say
The single available review rates the antibody 3 out of 5 stars. The reviewer used it for Western Blotting on mouse brain tissue at a 1:500 dilution and reported that the antibody works well in mouse brain tissue for this application. Overall, the feedback is positive regarding its performance in WB on mouse brain samples.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Tanusree (Verified Customer) (08-14-2019) | The antibody works good in mouse brain tissue in western blotting.
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