Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, HEK-293 cells, Raji cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15249-1-AP targets CDCA7 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4297 Product name: Recombinant human CDCA7 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 1-371 aa of BC027966 Sequence: MDARRVPQKDLRVKKNLKKFRYVKLISMETSSSSDDSCDSFASDNFANTRLQSVREGCRTRSQCRHSGPLRVAMKFPARSTRGATNKKAESRQPSENSVTDSNSDSEDESGMNFLEKRALNIKQNKAMLAKLMSELESFPGSFRGRHPLPGSDSQSRRPRRRTFPGVASRRNPERRARPLTRSRSRILGSLDALPMEEEEEEDKYMLVRKRKTVDGYMNEDDLPRSRRSRSSVTLPHIIRPVEEITEEELENVCSNSREKIYNRSLGSTCHQCRQKTIDTKTNCRNPDCWGVRGQFCGPCLRNRYGEEVRDALLDPNWHCPPCRGICNCSFCRQRDGRCATGVLVYLAKYHGFGNVHAYLKSLKQEFEMQA Predict reactive species |
| Full Name | cell division cycle associated 7 |
| Calculated Molecular Weight | 43 kDa |
| Observed Molecular Weight | 40-43 kDa |
| GenBank Accession Number | BC027966 |
| Gene Symbol | CDCA7 |
| Gene ID (NCBI) | 83879 |
| RRID | AB_2878119 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BWT1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CDCA7, also named as JP01, participates in MYC-mediated cell transformation and apoptosis. It plays a role as transcriptional regulator.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CDCA7 antibody 15249-1-AP | Download protocol |
| IHC protocol for CDCA7 antibody 15249-1-AP | Download protocol |
| IP protocol for CDCA7 antibody 15249-1-AP | Download protocol |
| WB protocol for CDCA7 antibody 15249-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CDCA7 Polyclonal antibody (catalog number 15249-1-AP) has 17 citations. These appear in journals including Cancer Discovery, Nucleic Acids Research, Science Advances, Journal of Experimental Medicine, PNAS, Cell Chemical Biology, Frontiers in Pharmacology, Oncology Research, Journal of Biological Chemistry, BMC Neuroscience, Genes, and Human Molecular Genetics. Research fields span epigenetics, chromatin remodeling, DNA methylation, ICF syndrome, and cancers (colorectal, gastric, ovarian, glioma, breast), plus ferroptosis, neurodevelopment, and inflammation.
| Species | Application | Title |
|---|---|---|
Cancer Discov Epigenetic and oncogenic inhibitors cooperatively drive differentiation and kill KRAS-mutant colorectal cancers | ||
Nucleic Acids Res The C-terminal 4CXXC-type zinc finger domain of CDCA7 recognizes hemimethylated DNA and modulates activities of chromatin remodeling enzyme HELLS | ||
Nucleic Acids Res The ZBTB24-CDCA7-HELLS axis suppresses the totipotent 2C-like reprogramming by maintaining Dux methylation and repression | ||
Sci Adv CDCA7-associated global aberrant DNA hypomethylation translates to localized, tissue-specific transcriptional responses | ||
Int J Biol Sci Transmissible Gastroenteritis Virus (TGEV) Infection Alters the Expression of Cellular MicroRNA Species That Affect Transcription of TGEV Gene 7. | ||
J Exp Med Loss of ZBTB24 impairs nonhomologous end-joining and class-switch recombination in patients with ICF syndrome. |
Reviews
What customers say
Users report mixed results with this CDCA7 antibody. One reviewer (5/5) successfully used it at 1:250 dilution for immunofluorescence on 293T cells overexpressing human CDCA7, observing clear nuclear localization. Another reviewer (3/5) tested it in Western blot, immunoprecipitation, and ChIP on fibroblasts and lymphoblastoid cells, noting the signal appeared at a lower-than-expected molecular weight and that the antibody performed poorly in ChIP. Overall, the antibody works well for IF but may have limitations in ChIP applications and signal specificity in WB/IP.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
XIAOBING (Verified Customer) (11-05-2019) | CDCA7 antibody was used to stain the 293T cells transfected with human CDCA7 plasmid. Overexpressed CDCA7 (Red) was localized in the nucleus (DAPI was used to label the nucleus).
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Guillaume (Verified Customer) (09-08-2019) | I used cells mutated for ZBTB24 which control CDCA7 expression to test the specificity of the antibody. In WB and IP, the signal seem to be lower than the expected size in kDa. This CDCA7 antibody is not very efficient in ChIP
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