Tested Applications
| Positive WB detected in | HepG2 cells, HeLa cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15273-1-AP targets POP4 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7387 Product name: Recombinant human POP4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-220 aa of BC004438 Sequence: MKSVIYHALSQKEANDSDVQPSGAQRAEAFVRAFLKRSTPRMSPQAREDQLQRKAVVLEYFTRHKRKEKKKKAKGLSARQRRELRLFDIKPEQQRYSLFLPLHELWKQYIRDLCSGLKPDTQPQMIQAKLLKADLHGAIISVTKSKCPSYVGITGILLQETKHIFKIITKEDRLKVIPKLNCVFTVETDGFISYIYGSKFQLRSSERSAKKFKAKGTIDL Predict reactive species |
| Full Name | processing of precursor 4, ribonuclease P/MRP subunit (S. cerevisiae) |
| Calculated Molecular Weight | 25 kDa |
| Observed Molecular Weight | 29 kDa |
| GenBank Accession Number | BC004438 |
| Gene Symbol | POP4 |
| Gene ID (NCBI) | 10775 |
| RRID | AB_2284033 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95707 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for POP4 antibody 15273-1-AP | Download protocol |
| WB protocol for POP4 antibody 15273-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
The product has received one review with a 5-star rating. The reviewer reported that the antibody "works well" for Western Blot applications, tested on JLat cells. Overall, the feedback is positive, indicating satisfactory performance in its intended use.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Arnaud (Verified Customer) (07-03-2025) | Works well
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