Tested Applications
| Positive WB detected in | mouse heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15300-1-AP targets NDUFA7 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7558 Product name: Recombinant human NDUFA7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-113 aa of BC003102 Sequence: MASATRLIQRLRNWASGHDLQGKLQLRYQEISKRTQPPPKLPVGPSHKLSNNYYCTRDGRRESVPPSIIMSSQKALVSGKPAESSAVAATEKKAVTPAPPIKRWELSSDQPYL Predict reactive species |
| Full Name | NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 7, 14.5kDa |
| Calculated Molecular Weight | 13 kDa |
| Observed Molecular Weight | 13 kDa |
| GenBank Accession Number | BC003102 |
| Gene Symbol | NDUFA7 |
| Gene ID (NCBI) | 4701 |
| RRID | AB_2878123 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95182 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NDUFA7(NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7) is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NDUFA7 antibody 15300-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NDUFA7 Polyclonal antibody (Cat# 15300-1-AP) has 3 total citations. These appear in Nature Communications, Antioxidants (Basel), and Brain Pathology. The associated research fields include mitochondrial glutathione transport and iron homeostasis linked to OXPHOS, oxidative phosphorylation inhibition and apoptosis via ROS induction, and mitochondrial activity in Parkinson's disease. All three studies used the antibody for Western blot in human samples.
| Species | Application | Title |
|---|---|---|
Nat Commun Combinatorial GxGxE CRISPR screen identifies SLC25A39 in mitochondrial glutathione transport linking iron homeostasis to OXPHOS. | ||
Antioxidants (Basel) The Novel Elemene Derivative, OMe-Ph-Elemene, Attenuates Oxidative Phosphorylation and Facilitates Apoptosis by Inducing Intracellular Reactive Oxygen Species | ||
Brain Pathol Mitochondrial activity in the frontal cortex area 8 and angular gyrus in Parkinson's disease and Parkinson's disease with dementia. |
