Tested Applications
| Positive WB detected in | K-562 cells, A549 cells, HeLa cells |
| Positive IP detected in | K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15329-1-AP targets RRS1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7030 Product name: Recombinant human RRS1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-365 aa of BC001811 Sequence: MEGQSVEELLAKAEQDEAEKLQRITVHKELELQFDLGNLLASDRNPPTGLRCAGPTPEAELQALARDNTQLLINQLWQLPTERVEEAIVARLPEPTTRLPREKPLPRPRPLTRWQQFARLKGIRPKKKTNLVWDEVSGQWRRRWGYQRARDDTKEWLIEVPGNADPLEDQFAKRIQAKKERVAKNELNRLRNLARAHKMQLPSAAGLHPTGHQSKEELGRAMQVAKVSTASVGRFQERLPKEKVPRGSGKKRKFQPLFGDFAAEKKNQLELLRVMNSKKPQLDVTRATNKQMREEDQEEAAKRRKMSQKGKRKGGRQGPGGKRKGGPPSQGGKRKGGLGGKMNSGPPGLGGKRKGGQRPGGKRRK Predict reactive species |
| Full Name | RRS1 ribosome biogenesis regulator homolog (S. cerevisiae) |
| Calculated Molecular Weight | 41 kDa |
| Observed Molecular Weight | 41 kDa |
| GenBank Accession Number | BC001811 |
| Gene Symbol | RRS1 |
| Gene ID (NCBI) | 23212 |
| RRID | AB_2301118 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15050 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for RRS1 antibody 15329-1-AP | Download protocol |
| WB protocol for RRS1 antibody 15329-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RRS1 Polyclonal antibody (Cat# 15329-1-AP) has 6 citations published in Nature Communications, Advanced Science, Science Advances, Biology Direct, Tissue and Cell, and BMC Medical Genomics. Research fields span prostate cancer treatment, male meiosis and m6A RNA modification, hepatocellular carcinoma, peroxisomal function and cholesterol metabolism, lung cancer ferroptosis, and ribosome biogenesis-related prognostic modeling. All cited applications are Western Blot in human or mouse samples.
| Species | Application | Title |
|---|---|---|
Nat Commun Targeting dual-specificity tyrosine phosphorylation-regulated kinase 2 with a highly selective inhibitor for the treatment of prostate cancer. | ||
Adv Sci (Weinh) m6A Reader hnRNPA2B1 Modulates Late Pachytene Progression in Male Meiosis Through Post-Transcriptional Control | ||
Sci Adv Genomic gain of RRS1 promotes hepatocellular carcinoma through reducing the RPL11-MDM2-p53 signaling.
| ||
Biol Direct Depletion of LONP2 unmasks differential requirements for peroxisomal function between cell types and in cholesterol metabolism | ||
Tissue Cell Silencing ribosome biogenesis regulator 1 homolog (RRS1) inhibits angiogenesis and cisplatin resistance of lung cancer cells by activating ferroptosis mediated by p53 pathway | ||
BMC Med Genomics Construction and verification of a prognostic model for prostate cancer based on ribosome biogenesis-related genes. |
