Tested Applications
| Positive WB detected in | A431 cells, Jurkat cells, HepG2 cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15338-1-AP targets MED6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7541 Product name: Recombinant human MED6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-246 aa of BC004106 Sequence: MAAVDIRDNLLGISWVDSSWIPILNSGSVLDYFSERSNPFYDRTCNNEVVKMQRLTLEHLNQMVGIEYILLHAQEPILFIIRKQQRQSPAQVIPLADYYIIAGVIYQAPDLGSVINSRVLTAVHGIQSAFDEAMSYCRYHPSKGYWWHFKDHEEQDKVRPKAKRKEEPSSIFQRQRVDALLLDLRQKFPPKFVQLKPGEKPVPVDQTKKEAEPIPETVKPEEKETTKNVQQTVSAKGPPEKRMRLQ Predict reactive species |
| Full Name | mediator complex subunit 6 |
| Calculated Molecular Weight | 28 kDa |
| Observed Molecular Weight | 28-32 kDa |
| GenBank Accession Number | BC004106 |
| Gene Symbol | MED6 |
| Gene ID (NCBI) | 10001 |
| RRID | AB_2142554 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75586 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MED6 is a part of mediator complex. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MED6 antibody 15338-1-AP | Download protocol |
| WB protocol for MED6 antibody 15338-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MED6 Polyclonal antibody (Cat# 15338-1-AP) has 1 citation. It appears in Molecular Cell (2024), in a study on an IDR-dependent mechanism for nuclear receptor control of Mediator interaction with RNA polymerase II. The research field is transcriptional regulation and nuclear receptor signaling, with the antibody used for Western blot in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Mol Cell An IDR-dependent mechanism for nuclear receptor control of Mediator interaction with RNA polymerase II |
Reviews
What customers say
Both reviewers used this antibody for Western Blot. One reported a clear band at the expected molecular weight in mouse tissue at 1:1000 dilution, though occasional non-specific bands were observed, rating it 4/5. The other used it at 1:500 on embryo heart tissue and rated it 5/5, describing the result as good. Overall, users find it effective for WB with strong specific signal, while noting minor background issues at certain dilutions.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
anh (Verified Customer) (04-18-2022) | claer band on target molecular weight , but sometimes have some unspecific band on membrane other size
|
SITING (Verified Customer) (05-25-2021) | good
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