Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, MCF-7 cells, PC-3 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15342-1-AP targets MRPL9 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7545 Product name: Recombinant human MRPL9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-267 aa of BC004517 Sequence: MAAPVVTAPGRALLRAGAGRLLRGGVQELLRPRHEGNAPDLACNFSLSQNRGTVIVERWWKVPLAGEGRKPRLHRRHRVYKLVEDTKHRPKENLELILTQSVENVGVRGDLVSVKKSLGRNRLLPQGLAVYASPENKKLFEEEKLLRQEGKLEKIQTKAGEATVKFLKSCRLEVGMKNNVKWELNPEIVARHFFKNLGVVVAPHTLKLPEEPITRWGEYWCEVTVNGLDTVRVPMSVVNFEKPKTKRYKYWLAQQAAKAMAPTSPQI Predict reactive species |
| Full Name | mitochondrial ribosomal protein L9 |
| Calculated Molecular Weight | 30 kDa |
| Observed Molecular Weight | 26-28 kDa |
| GenBank Accession Number | BC004517 |
| Gene Symbol | MRPL9 |
| Gene ID (NCBI) | 65005 |
| RRID | AB_2146214 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BYD2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MRPL9 antibody 15342-1-AP | Download protocol |
| IHC protocol for MRPL9 antibody 15342-1-AP | Download protocol |
| WB protocol for MRPL9 antibody 15342-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MRPL9 Polyclonal antibody (Cat# 15342-1-AP) has 7 total citations. These appear in journals including Nature Structural & Molecular Biology, PNAS, International Journal of Molecular Sciences, Human Genetics, Reproduction, Human Molecular Genetics, and Frontiers in Surgery. The associated research fields span mitochondrial ribosome assembly and biogenesis, aging and T cell dysfunction, cancer (papillary thyroid cancer and hepatocellular carcinoma), genetic disorders (premature ovarian insufficiency, pediatric mitochondrial disease), and reproductive pathology (preeclampsia).
| Species | Application | Title |
|---|---|---|
Proc Natl Acad Sci U S A Cryoelectron tomography reveals an age-related decline in mitoribosomes that contributes to T cell dysfunction in older individuals | ||
Int J Mol Sci Interaction of MRPL9 and GGCT Promotes Cell Proliferation and Migration by Activating the MAPK/ERK Pathway in Papillary Thyroid Cancer | ||
Reproduction MRPL13 deficiency triggers trophoblast mitochondrial unfolded protein response in early-onset preeclampsia. | ||
Hum Genet Deficiency of the mitochondrial ribosomal subunit, MRPL50, causes autosomal recessive syndromic premature ovarian insufficiency | ||
Hum Mol Genet Multi-omics identifies large mitoribosomal subunit instability caused by pathogenic MRPL39 variants as a cause of pediatric onset mitochondrial disease |
