Tested Applications
| Positive WB detected in | mouse heart tissue, mouse skeletal muscle tissue, rat heart tissue |
| Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15396-1-AP targets MYL10 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7594 Product name: Recombinant human MYL10 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-226 aa of BC002778 Sequence: MLLRLVSNSWPQVILPPRPPKVLGLQAPRRARKRAEGTASSNVFSMFDQSQIQEFKESLALSPRLERNGMISAHCNLCLTGSSNSPASASQAFTIMDQNRDGFIDKEDLRDTFAALGRINVKNEELEAMVKEAPGPINFTVFLTMFGEKLKGTDPEETILHAFKVFDTEGKGFVKADVIKEKLMTQADRFSEEEVKQMFAAFPPDVCGNLDYRNLCYVITHGEEKD Predict reactive species |
| Full Name | myosin, light chain 10, regulatory |
| Calculated Molecular Weight | 25 kDa |
| Observed Molecular Weight | 20 kDa |
| GenBank Accession Number | BC002778 |
| Gene Symbol | MYL10 |
| Gene ID (NCBI) | 93408 |
| RRID | AB_3741865 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BUA6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
In non-muscle cells, MLCs are either regulatory (RLC) or essential ELCs. The human RLC gene family encompasses MYL2, MYL5, MYL9, MYL10, MYL11, MYL12A, and MYL12B , with the ELC gene family including MYL1 , MYL3, MYL4, and MYL6(PMID: 39768172). MYL10, also known as PLRLC, encodes for an RLC that might be a lymphocyte specific precursor. MYL10 is a regulatory myosin light chain that is expressed in pre-B cells from adult bone marrow, but not those from fetal liver, upon exposure to IL-7(PMID: 1628631).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MYL10 antibody 15396-1-AP | Download protocol |
| WB protocol for MYL10 antibody 15396-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MYL10 Polyclonal antibody (Cat# 15396-1-AP) has a total of 1 citation. The cited publication appeared in Microvascular Research (Impact Factor: 2.9), published in 2026. The study investigated PEAR1/EAF1 deficiency and its role in impairing aspirin responsiveness in atherosclerotic endothelium, placing the research in the fields of cardiovascular biology, endothelial function, and aspirin resistance mechanisms. The antibody was used for immunofluorescence (IF) in human samples.
| Species | Application | Title |
|---|---|---|
Microvasc Res PEAR1/EAF1 deficiency impairs aspirin responsiveness in atherosclerotic endothelium: A novel mechanism of atypical aspirin resistance. |
