Tested Applications
| Positive WB detected in | mouse brain tissue |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15410-1-AP targets FAPP2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7677 Product name: Recombinant human PLEKHA8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC002838 Sequence: MEGVLYKWTNYLSGWQPRWFLLCGGILSYYDSPEDAWKGCKGSIQMAVCEIQVHSVDNTRMDLIIPGEQYFYLKARSVAERQRWLVALGSAKACLTDSRTQKEKEFAENTENLKTKMSELRLYCDLLVQQVDKTKEVTTTGVSNSEEGIDVGTLLKSTCNTFLKTLEECMQIANAAFTSELLYRTPPGSPQLAMLKSSKMKHPIIPIHNSLERQMELSTCENGSLNMEINGEEEILMKNKNSLYLKSAEIDCSISSEENTDDNITVQGEIRKEDGMENLKNHDNNLTQSGSDSSCSPECLWEEGKEVIPTFFSTMNTSFSDIELLEDSGIPTEAFLASCYAVVPVLDKLG Predict reactive species |
| Full Name | pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 8 |
| Calculated Molecular Weight | 58 kDa |
| Observed Molecular Weight | 49-58 kDa, 65 kDa |
| GenBank Accession Number | BC002838 |
| Gene Symbol | FAPP2 |
| Gene ID (NCBI) | 84725 |
| RRID | AB_2166928 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96JA3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for FAPP2 antibody 15410-1-AP | Download protocol |
| WB protocol for FAPP2 antibody 15410-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Anti-FAPP2 antibody (Cat# 15410-1-AP) has a total of 5 citations. These appear in Hematology Oncology, Experimental Cell Research, Reproduction, bioRxiv, and eLife. The associated research fields include T-cell acute lymphoblastic leukemia and PI3K/AKT/mTOR signaling, colon cancer and Wnt pathway activation, reproductive biology (missed abortion), spatiotemporal proteomics of NLRP3 inflammasome responses, and sphingomyelin synthesis via ORP9-ORP11 dimer function.
| Species | Application | Title |
|---|---|---|
Hematol Oncol Downregulation of FAPP2 gene induces cell autophagy and inhibits PI3K/AKT/mTOR pathway in T-cell acute lymphoblastic leukemia
| ||
Exp Cell Res FAPP2 promotes tumor cell growth in human colon cancer through activation of Wnt signaling.
| ||
