DLC1 Polyclonal antibody

DLC1 Polyclonal Antibody for IHC, IF/ICC, IP, ELISA

Cat No. 15460-1-AP

Publications (3)

Review Product

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

ARHGAP7, deleted in liver cancer 1, DLC 1, DLC-1, HP

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Filter: AllIPIHCIF/ICC

IP experiment of mouse lung using 15460-1-AP
IP result of anti-DLC1 (IP:15460-1-AP, 4ug; Detection:15460-1-AP 1:300) with mouse lung tissue lysate 2400ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human spleen using 15460-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 15460-1-AP (DLC1 antibody) at dilution of 1:100 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human spleen using 15460-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 15460-1-AP (DLC1 antibody) at dilution of 1:100 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse small intestine using 15460-1-AP
Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 15460-1-AP (DLC1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human placenta using 15460-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15460-1-AP (DLC1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse kidney using 15460-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15460-1-AP (DLC1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse ovary using 15460-1-AP
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 15460-1-AP (DLC1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver using 15460-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 15460-1-AP (DLC1 antibody) at dilution of 1:100 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver using 15460-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 15460-1-AP (DLC1 antibody) at dilution of 1:100 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HepG2 using 15460-1-AP
Immunofluorescent analysis of HepG2 cells, using DLC1 antibody 15460-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for DLC1 in ?

Overview

Tested Applications
Product Information
Publications (3)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive IP detected in	mouse lung tissue
Positive IHC detected in	human spleen tissue, human placenta tissue, human liver tissue, mouse small intestine tissue, mouse kidney tissue, mouse ovary tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15460-1-AP targets DLC1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7732 Product name: Recombinant human DLC1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1179-1528 aa of BC054511 Sequence: KDQRLQAIKAAIMLLPDENREVLQTLLYFLSDVTAAVKENQMTPTNLAVCLAPSLFHLNTLKRENSSPRVMQRKQSLGKPDQKDLNENLAATQGLAHMIAECKKLFQVPEEMSRCRNSYTEQELKPLTLEALGHLGNDDSADYQHFLQDCVDGLFKEVKEKFKGWVSYSTSEQAELSYKKVSEGPPLRLWRSVIEVPAVPEEILKRLLKEQHLWDVDLLDSKVIEILDSQTEIYQYVQNSMAPHPARDYVVLRTWRTNLPKGACALLLTSVDHDRAPVVGVRVNVLLSRYLIEPCGPGKSKLTYMCRVDLRGHMPEWYTKSFGHLCAAEVVKIRDSFSNQNTETKDTKSR Predict reactive species
Full Name	deleted in liver cancer 1
Calculated Molecular Weight	171 kDa
GenBank Accession Number	BC054511
Gene Symbol	DLC1
Gene ID (NCBI)	10395
RRID	AB_2246077
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96QB1
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for DLC1 antibody 15460-1-AP	Download protocol
IHC protocol for DLC1 antibody 15460-1-AP	Download protocol
IP protocol for DLC1 antibody 15460-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The DLC1 polyclonal antibody (Cat# 15460-1-AP) has 3 total citations, appearing in Cancer Research, Biomaterials, and Journal of Sexual Medicine. The associated research fields span oncology (cholangiocarcinoma progression driven by KRAS/CK2), biomechanics (cell-matrix mechanotransduction and 3D remodeling), and sexual medicine (erectile dysfunction under hypoandrogenic conditions). Cited applications include WB, IHC, and IF, with reactivity in human and rat.

Species	Application	Title
human	IF	Cancer Res Mutant KRAS and CK2 Cooperatively Stimulate SLC16A3 Activity to Drive Intrahepatic Cholangiocarcinoma Progression Authors - Ran Chen View Article
human	WB	Biomaterials Stiffness assisted cell-matrix remodeling trigger 3D mechanotransduction regulatory programs Authors - Anna L Kersey View Article
rat	WB,IHC	J Sex Med Hypoandrogenic state inhibits erectile function in rats via miR-200a-3p Authors - Yuan Wang View Article