Tested Applications
| Positive WB detected in | HEK-293 cells, human kidney tissue |
| Positive IP detected in | HEK-293 cells, L02 cells |
| Positive IHC detected in | human stomach cancer tissue, human heart tissue, mouse stomach tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15472-1-AP targets VPS24 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7776 Product name: Recombinant human VPS24 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-222 aa of BC004419 Sequence: MGLFGKTQEKPPKELVNEWSLKIRKEMRVVDRQIRDIQREEEKVKRSVKDAAKKGQKDVCIVLAKEMIRSRKAVSKLYASKAHMNSVLMGMKNQLAVLRVAGSLQKSTEVMKAMQSLVKIPEIQATMRELSKEMMKAGIIEEMLEDTFESMDDQEEMEEEAEMEIDRILFEITAGALGKAPSKVTDALPEPEPPGAMAASEDEEEEEEALEAMQSRLATLRS Predict reactive species |
| Full Name | vacuolar protein sorting 24 homolog (S. cerevisiae) |
| Calculated Molecular Weight | 25 kDa |
| Observed Molecular Weight | 29 kDa |
| GenBank Accession Number | BC004419 |
| Gene Symbol | VPS24 |
| Gene ID (NCBI) | 51652 |
| RRID | AB_2217102 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y3E7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for VPS24 antibody 15472-1-AP | Download protocol |
| IHC protocol for VPS24 antibody 15472-1-AP | Download protocol |
| IP protocol for VPS24 antibody 15472-1-AP | Download protocol |
| WB protocol for VPS24 antibody 15472-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The VPS24 Polyclonal antibody (Cat# 15472-1-AP) has 7 citations published in journals including Signal Transduction and Targeted Therapy, Nature Cell Biology, International Journal of Oncology, Frontiers in Immunology, Journal of Cell Biology, Journal of Extracellular Biology, and Annals of Surgical Oncology. Research fields span pancreatic carcinogenesis, ESCRT-III-dependent membrane trafficking, hepatocellular carcinoma and pyroptosis, exosome tetraspanin sorting, extracellular vesicle fusion in colorectal cancer, and CHMP3 functional roles in cancer.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Serine/threonine/tyrosine kinase 1 drives pancreatic carcinogenesis via GSK3β sequestration-mediated Wnt/β-catenin pathway hyperactivation. | ||
Nat Cell Biol Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation. | ||
Int J Oncol CHMP3 promotes the progression of hepatocellular carcinoma by inhibiting caspase‑1‑dependent pyroptosis
| ||
Front Immunol Prognostic model construction and immune microenvironment analysis of pyroptosis-related genes in hepatocellular carcinoma based on single-cell RNA sequencing | ||
J Cell Biol ALIX- and ESCRT-III-dependent sorting of tetraspanins to exosomes.
| ||
J Extracell Biol RAB31 drives extracellular vesicle fusion and cancer-associated fibroblast formation leading to oxaliplatin resistance in colorectal cancer |
