Tested Applications
| Positive WB detected in | HepG2 cells, A549 cells, Jurkat cells, HeLa cells |
| Positive IP detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 4 publications below |
| IP | See 1 publications below |
Product Information
15529-1-AP targets MTX1 in WB, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7685 Product name: Recombinant human MTX1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 75-435 aa of BC001906 Sequence: YVGHLWMGRRPPSPEARGPVPRSSAASRARRSLASPGISPGPLTATIGGAVAGGGPRQGRAEAHKEVFPGQRVGKMAAPMELFCWSGGWGLPSVDLDSLAVLTYARFTGAPLKVHKISNPWQSPSGTLPALRTSHGEVISVPHKIITHLRKEVHTFWIDTKNYVEVTRKWYAEAMPFPLNFFLPGRMQRQYMERLQLLTGEHRPEDEEELEKELYREARECLTLLSQRLGSQKFFFGDAPASLDAFVFSYLALLLQAKLPSGKLQVHLRGLHNLCAYCTHILSLYFPWDGAEVPPQRQTPAGPETEEEPYRRRNQILSVLAGLAAMVGYALLSGIVSIQRATPARAPGTRTLGMAEEDEEE Predict reactive species |
| Full Name | metaxin 1 |
| Calculated Molecular Weight | 51 kDa |
| Observed Molecular Weight | 35 kDa |
| GenBank Accession Number | BC001906 |
| Gene Symbol | MTX1 |
| Gene ID (NCBI) | 4580 |
| RRID | AB_2148416 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13505 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
METAXINs are proteins located in the outer membrane of the mammalian mitochondrion. METAXIN 1 was characterized firstly and was found to play a role in the import of mitochondrial precursor proteins. It may also play a role in the assembly of b-barrel proteins into the outer membrane METAXIN1 was reported to be essential for survival of the postimplantation mouse embryo. METAXIN 1 has multiple transcripts. Several isoforms of METAXIN 1 exist due to the alternative splicing, with the predicted MW of 51, 48 and 36 kDa, respectively. Metaxin 1 interacts with metaxin 2, a novel related protein associated with the mammalian mitochondrial outer membrane and form complex in the mitochondrion outer membrane.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for MTX1 antibody 15529-1-AP | Download protocol |
| WB protocol for MTX1 antibody 15529-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Biol Sci Genome-scale CRISPRa screening identifies MTX1 as a contributor for sorafenib resistance in hepatocellular carcinoma by augmenting autophagy.
| ||
Hepatology Loss of Sam50 in hepatocytes induces cardiolipin-dependent mitochondrial membrane remodeling to trigger mtDNA release and liver injury | ||
J Cell Sci Myo19 tethers mitochondria to endoplasmic reticulum-associated actin to promote mitochondrial fission
| ||
NPJ Parkinsons Dis Disruption of axonal transport in Parkinson's disease: the role of pathological α-Syn and AMPK/p38 MAPK signaling |
