Tested Applications
| Positive WB detected in | recombinant protein |
| Positive IHC detected in | mouse cerebellum tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15569-1-AP targets AGBL5/CCP5 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7889 Product name: Recombinant human AGBL5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 466-816 aa of BC007415 Sequence: SLNSAHFDFQGCNFSEKNMYARDRRDGQSKEGSGRVAIYKASGIIHSYTLECNYNTGRSVNSIPAACHDNGRASPPPPPAFPSRYTVELFEQVGRAMAIAALDMAECNPWPRIVLSEHSSLTNLRAWMLKHVRNSRGLSSTLNVGVNKKRGLRTPPKSHNGLPVSCSENTLSRARSFSTGTSAGGSSSSQQNSPQMKNSPSFPFHGSRPAGLPGLGSSTQKVTHRVLGPVREPRSQDRRRQQQPLNHRPAGSLAPSPAPTSSGPASSHKLGSCLLPDSFNIPGSSCSLLSSGDKPEAVMVIGKGLLGTGARMPCIKTRLQTCPRRVSARRGPGFPRLGPGWAGAHRRLAEG Predict reactive species |
| Full Name | ATP/GTP binding protein-like 5 |
| Calculated Molecular Weight | 98 kDa |
| GenBank Accession Number | BC007415 |
| Gene Symbol | AGBL5/CCP5 |
| Gene ID (NCBI) | 60509 |
| RRID | AB_2878151 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8NDL9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for AGBL5/CCP5 antibody 15569-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
The single review for this product (1-star rating) reports unsuccessful Western Blot results. The user tested the antibody at a 1:1000 dilution on U2OS cells, both transfected with a CCP5-GFP plasmid and for endogenous CCP5 detection, but was unable to obtain a clear signal in either case. Overall, the reviewer found the antibody ineffective for their intended application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Jessica (Verified Customer) (10-21-2021) | U2OS cells transfected with a plasmid coding for CCP5-GFP Do not succed to have a nice signal for CCP5-GFP, nor for endogenous CCP5.
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