Tested Applications
| Positive WB detected in | HL-60 cells, U-937 cells, Raji cells |
| Positive IP detected in | Raji cells |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15593-1-AP targets LSP1 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7944 Product name: Recombinant human LSP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-339 aa of BC001785 Sequence: MAEASSDPGAEEREELLGPTAQWSVEDEEEAVHEQCQHERDRQLQAQDEEGGGHVPERPKQEMLLSLKPSEAPELDEDEGFGDWSQRPEQRQQHEGAQGTLDSGEPPQCRSPEGEQEDRPGLHAYEKEDSDEVHLEELSLSKEGPGPEDTVQDNLGAAGAEEEQEEHQKCQQPRTPSPLVLEGTIEQSSPPLSPTTKLIDRTESLNRSIEKSNSVKKSQPDLPISKIDQWLEQYTQAIETAGRTPKLARQASIELPSMAVASTKSRWETGEVQAQSAAKTPSCKDIVAGDMSKKSLWEQKGGSKTSSTIKSTPSGKRYKFVATGHGKYEKVLVEGGPAP Predict reactive species |
| Full Name | lymphocyte-specific protein 1 |
| Calculated Molecular Weight | 37 kDa |
| Observed Molecular Weight | 52 kDa |
| GenBank Accession Number | BC001785 |
| Gene Symbol | LSP1 |
| Gene ID (NCBI) | 4046 |
| RRID | AB_2250134 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P33241 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Lymphocyte-specific protein 1(LSP1), also known as leukocyte-specific protein 1, is an F-actin binding and cytoskeleton associated protein. The protein is expressed in lymphocytes, neutrophils, and macrophages. LSP1 is a substrate for mitogen-activated protein kinase (MAPK)-activated protein kinase 2 and protein kinase C. The implication of LSP1 in diverse biological processes, such as inflammation and malignancy, suggests the major regulatory role of LSP1 in signal transduction(PMID: 38254711).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for LSP1 antibody 15593-1-AP | Download protocol |
| IP protocol for LSP1 antibody 15593-1-AP | Download protocol |
| WB protocol for LSP1 antibody 15593-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The LSP1 Polyclonal antibody (Cat# 15593-1-AP) has 1 citation to date. It was cited in a study published in FASEB Journal (2025), which developed a lactylation-based diagnostic model for identifying molecular subtypes and therapeutic targets in dilated cardiomyopathy. The antibody was used in Western blot and IHC applications on human samples. The research field encompasses cardiomyopathy, post-translational modifications (lactylation), and molecular diagnostics.
| Species | Application | Title |
|---|---|---|
FASEB J A Lactylation-Based Diagnostic Model Reveals Molecular Subtypes and Therapeutic Targets in Dilated Cardiomyopathy. |
