Tested Applications
| Positive WB detected in | NIH/3T3 cells, Raji cells, Ramos cells, THP-1 cells |
| Positive IF-P detected in | human tonsillitis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15642-1-AP targets AID in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8136 Product name: Recombinant human AID protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-198 aa of BC006296 Sequence: MDSLLMNRRKFLYQFKNVRWAKGRRETYLCYVVKRRDSATSFSLDFGYLRNKNGCHVELLFLRYISDWDLDPGRCYRVTWFTSWSPCYDCARHVADFLRGNPNLSLRIFTARLYFCEDRKAEPEGLRRLHRAGVQIAIMTFKDYFYCWNTFVENHERTFKAWEGLHENSVRLSRQLRRILLPLYEVDDLRDAFRTLGL Predict reactive species |
| Full Name | activation-induced cytidine deaminase |
| Calculated Molecular Weight | 198 aa, 24 kDa |
| Observed Molecular Weight | 24 kDa |
| GenBank Accession Number | BC006296 |
| Gene Symbol | AIDA/AID |
| Gene ID (NCBI) | 57379 |
| RRID | AB_2224236 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9GZX7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
AID is essential to all three genetic alterations required for antigen-specific immunoglobulin: class switch recombination (CSR), somatic bypermutation (SHM), and gene conversion. Expression of AID is normally restricted to B cells in the GC, where SHM and CSR occur. It is likely these represent AID monomer and homodimer that western blot detected two bands in purified AID from nuclear extracts, one at 52 kDa and the other at 26 kDa. (PMID:16280388)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for AID antibody 15642-1-AP | Download protocol |
| WB protocol for AID antibody 15642-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The AID Polyclonal antibody (Cat# 15642-1-AP) has 1 total citation. It appears in Cell (IF 45.1), in a study titled "Co-option of retrotransposons promotes antibody diversification" by Max C. Lauring (2026). The research field is immunology, specifically antibody diversification and B cell biology.
| Species | Application | Title |
|---|---|---|
