Tested Applications
| Positive WB detected in | HeLa cells, PC-3 cells |
| Positive IHC detected in | rat stomach tissue, human placenta tissue, human rectal cancer tissue, mouse colon tissue, mouse pancreas tissue, mouse stomach tissue, rat kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15677-1-AP targets LZTS2 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8145 Product name: Recombinant human LZTS2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 319-669 aa of BC006212 Sequence: PSDEALLHCVLEGKLRDREAELQQLRDSLDENEATMCQAYEERQRHWQREREALREDCAAQAQRAQRAQQLLQLQVFQLQQEKRQLQDDFAQLLQEREQLERRCATLEREQRELGPRLEETKWEVCQKSGEISLLKQQLKESQAELVQKGSELVALRVALREARATLRVSEGRARGLQEAARARELELEACSQELQRHRQEAEQLREKAGQLDAEAAGLREPPVPPATADPFLLAESDEAKVQRAAAGVGGSLRAQVERLRVELQRERRRGEEQRDSFEGERLAWQAEKEQVIRYQKQLQHNYIQMYRRNRQLEQELQQLSLELEARELADLGLAEQAPCICLEEITATEI Predict reactive species |
| Full Name | leucine zipper, putative tumor suppressor 2 |
| Calculated Molecular Weight | 73 kDa |
| Observed Molecular Weight | 70-80 kDa |
| GenBank Accession Number | BC006212 |
| Gene Symbol | LZTS2 |
| Gene ID (NCBI) | 84445 |
| RRID | AB_2878166 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BRK4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LZTS2, also named as KIAA1813 and LAPSER1, is a novel b-Catenin-interacting protein and it regulates the nuclear export of b-Catenin. LZTS2 is a negative regulator of katanin-mediated microtubule severing and release from the centrosome. It is required for central spindle formation and the completion of cytokinesis.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for LZTS2 antibody 15677-1-AP | Download protocol |
| WB protocol for LZTS2 antibody 15677-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-LZTS2 antibody (Cat# 15677-1-AP) has 11 citations published in journals including Nature Communications, Cell Death & Disease, Cancer Letters, Oncogene, Biochemical Pharmacology, Journal of Cell Biology, Biology Direct, Cell Signaling, Cytoskeleton, and bioRxiv. Research fields span cancer biology (colorectal, nasopharyngeal, hepatocellular, lung), Wnt and PI3K/AKT signaling, ubiquitination, cell cycle regulation, cytokinesis, and mechanobiology.
| Species | Application | Title |
|---|---|---|
Nat Commun Proximity proteomics identifies PAK4 as a component of Afadin-Nectin junctions. | ||
Cell Death Dis RRP15 deficiency induces ribosome stress to inhibit colorectal cancer proliferation and metastasis via LZTS2-mediated β-catenin suppression | ||
Cell Death Dis SPOP and HAUSP bidirectionally regulate LZTS2 ubiquitination to modulate the Wnt pathway.
| ||
Cancer Lett LZTS2 inhibits PI3K/AKT activation and radioresistance in nasopharyngeal carcinoma by interacting with p85.
| ||
Oncogene β-Trcp and CK1δ-mediated degradation of LZTS2 activates PI3K/AKT signaling to drive tumorigenesis and metastasis in hepatocellular carcinoma.
| ||
Biochem Pharmacol EBV-encoded miRNAs BHRF1-1 and BART2-5p aggravate post- transplant lymphoproliferative disorder via LZTS2-PI3K-AKT axis |
