Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells |
| Positive IP detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15747-1-AP targets NPHP5/IQCB1 in WB, IF, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8203 Product name: Recombinant human NPHP5,IQCB1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-350 aa of BC005806 Sequence: MKPTGTDPRILSIAAEVAKSPEQNVPVILLKLKEIINITPLGSSELKKIKQDIYCYDLIQYCLLVLSQDYSRIQGGWTTISQLTQILSHCCVGLEPGEDAEEFYNELLPSAAENFLVLGRQLQTCFINAAKAEEKDELLHFFQIVTDSLFWLLGGHVELIQNVLQSDHFLHLLQADNVQIGSAVMMMLQNILQINRSKRSKMLLEINRQKEEEDLKLQLQLQRQRAMRLSRELQLSMLEIVHPGQVEKHYREMEEKSALIIQKHWRGYRERKNFHQQRQSLIEYKAAVTLQRAALKFLAKYRKKKKLFAPWRGLQELTDARRVELKKRVDDYVRRHLGSPMSDVVSRELH Predict reactive species |
| Full Name | IQ motif containing B1 |
| Calculated Molecular Weight | 598 aa, 69 kDa |
| Observed Molecular Weight | 69 kDa |
| GenBank Accession Number | BC005806 |
| Gene Symbol | IQCB1 |
| Gene ID (NCBI) | 9657 |
| RRID | AB_2878179 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15051 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IQCB1, also known as NPHP5, is a nephrocystin protein that interacts with calmodulin and the retinitis pigmentosa GTPase regulator protein. It has a central coiled-coil region and two calmodulin-binding IQ domains. Localized to the primary cilia of renal epithelial cells and connecting cilia of photoreceptor cells, IQCB1 is thought to play a role in ciliary function. Mutations in this gene result in Senior-Loken syndrome type 5, a juvenile disorder characterized by defects in the waste filtering system of the kidney, as well as retinal degradation.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for NPHP5/IQCB1 antibody 15747-1-AP | Download protocol |
| WB protocol for NPHP5/IQCB1 antibody 15747-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NPHP5/IQCB1 Polyclonal antibody (Cat# 15747-1-AP) has 2 citations. These appear in Human Molecular Genetics and Molecular Biology of the Cell. The cited research focuses on ciliogenesis and ciliary gating at the vertebrate transition zone, specifically examining NPHP5's interaction with Cep290 and the role of Rpgrip1l in regulating Cep290 levels.
| Species | Application | Title |
|---|---|---|
Hum Mol Genet Pathogenic NPHP5 mutations impair protein interaction with Cep290, a prerequisite for ciliogenesis. | ||
Mol Biol Cell Rpgrip1l controls ciliary gating by ensuring the proper amount of Cep290 at the vertebrate transition zone. |
