Tested Applications
| Positive WB detected in | HeLa cells |
| Positive IHC detected in | mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15766-1-AP targets DUSP23 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8402 Product name: Recombinant human DUSP23 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-150 aa of BC001140 Sequence: MGVQPPNFSWVLPGRLAGLALPRLPAHYQFLLDLGVRHLVSLTERGPPHSDSCPGLTLHRLRIPDFCPPAPDQIDRFVQIVDEANARGEAVGVHCALGFGRTGTMLACYLVKERGLAAGDAIAEIRRLRPGSIETYEQEKAVFQFYQRTK Predict reactive species |
| Full Name | dual specificity phosphatase 23 |
| Calculated Molecular Weight | 17 kDa |
| Observed Molecular Weight | 15 kDa |
| GenBank Accession Number | BC001140 |
| Gene Symbol | DUSP23 |
| Gene ID (NCBI) | 54935 |
| RRID | AB_3085472 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BVJ7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for DUSP23 antibody 15766-1-AP | Download protocol |
| WB protocol for DUSP23 antibody 15766-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The DUSP23 Polyclonal antibody (catalog number 15766-1-AP) has a total of 1 citation. It appears in the journal Biomaterials (impact factor 13.6), in a 2026 study on modeling calcific aortic valve disease using engineered human valve tissues, which identified SAMHD1 as a therapeutic target. The associated research field is cardiovascular disease, specifically calcific aortic valve disease. The citation used the antibody for immunofluorescence (IF) in human samples.
| Species | Application | Title |
|---|---|---|
Biomaterials Modeling calcific aortic valve disease with engineered human valve tissues identifies SAMHD1 as a therapeutic target. |
Reviews
What customers say
The single review for this product is a 5-star rating from a user who tested it for immunohistochemistry and immunofluorescence on rat frozen muscle tissue. They tried dilutions ranging from 1/50 to 1/200 and found that 1/50 produced the strongest signal with low background. Pepsin was used as the antigen retrieval method. Overall, the reviewer was satisfied with the antibody's performance, noting clear and specific staining at the recommended dilution.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Mikhail (Verified Customer) (10-02-2026) | Tried 1/50-1/200 dilutions, 1/50 gave stronger signal with low background. Pepsin antigen retriever.
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