Tested Applications
| Positive WB detected in | HeLa cells, mouse liver tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse testis tissue, human kidney tissue, rat testis tissue, rat kidney tissue, human urothelial carcinoma tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15767-1-AP targets NQO2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8403 Product name: Recombinant human NQO2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-231 aa of BC006096 Sequence: MAGKKVLIVYAHQEPKSFNGSLKNVAVDELSRQGCTVTVSDLYAMNFEPRATDKDITGTLSNPEVFNYGVETHEAYKQRSLASDITDEQKKVREADLVIFQFPLYWFSVPAILKGWMDRVLCQGFAFDIPGFYDSGLLQGKLALLSVTTGGTAEMYTKTGVNGDSRYFLWPLQHGTLHFCGFKVLAPQISFAPEIASEEERKGMVAAWSQRLQTIWKEEPIPCTAHWHFGQ Predict reactive species |
| Full Name | NAD(P)H dehydrogenase, quinone 2 |
| Calculated Molecular Weight | 231 aa, 26 kDa |
| Observed Molecular Weight | 26 kDa |
| GenBank Accession Number | BC006096 |
| Gene Symbol | NQO2 |
| Gene ID (NCBI) | 4835 |
| RRID | AB_2236213 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P16083 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NQO2, also named as QR2 and NMOR2, belongs to the NAD(P)H dehydrogenase (quinone) family. It serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. The cytosolic quinone oxidoreductases NQO1 and NQO2 protect cells against oxidative stress by detoxifying quinones and preventing redox cycling. NQO1 and NQO2 are important endogenous factors in regulation of immune response and autoimmunity.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for NQO2 antibody 15767-1-AP | Download protocol |
| IHC protocol for NQO2 antibody 15767-1-AP | Download protocol |
| IP protocol for NQO2 antibody 15767-1-AP | Download protocol |
| WB protocol for NQO2 antibody 15767-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NQO2 antibody (Cat# 15767-1-AP) has 11 citations spanning journals including J Exp Clin Cancer Res, Free Radic Biol Med, FASEB J, Sci Rep, ACS Chem Biol, Antioxidants, J Neural Transm, J Proteome Res, J Appl Toxicol, Life Sci Alliance, and Cell Physiol Biochem. Research fields cover cancer therapy, autophagy, Parkinson's disease/neuroprotection, ferroptosis, pulmonary hypertension, vascular biology, and nephrotoxicity, with applications primarily in WB and IHC across human, mouse, and rat species.
| Species | Application | Title |
|---|---|---|
J Exp Clin Cancer Res PRX1 knockdown potentiates vitamin K3 toxicity in cancer cells: a potential new therapeutic perspective for an old drug. | ||
Antioxidants (Basel) Apigenin and Luteolin Regulate Autophagy by Targeting NRH-Quinone Oxidoreductase 2 in Liver Cells. | ||
Free Radic Biol Med Astragalin induces immunogenic cell death in liver cancer by targeting NQO2 to promote ROS-mediated endoplasmic reticulum stress pathway | ||
Sci Rep Autophagy and neuroprotection in astrocytes exposed to 6-hydroxydopamine is negatively regulated by NQO2: relevance to Parkinson's disease
| ||
J Neural Transm (Vienna) The specific NQO2 inhibitor, S29434, only marginally improves the survival of dopamine neurons in MPTP-intoxicated mice | ||
FASEB J FRK exerts oncogenic effects by targeting NQO2 via exosomal miR-9-3p to regulate mitochondrial function |
Reviews
What customers say
The single review for this product is highly positive, rated 5 stars. The reviewer used it for Western Blot at a 1:2000 dilution, testing it on Hela cells, mouse liver, and mouse brain tissue. They described it as a "great antibody for WB," indicating strong performance and reliability in their application. Overall, the feedback reflects satisfaction with the antibody's effectiveness in Western Blot experiments.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Hua (Verified Customer) (07-08-2026) | Great antibody for WB.
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