Tested Applications
| Positive WB detected in | Jurkat cells, K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15991-1-AP targets TRPV2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8864 Product name: Recombinant human TRPV2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC018926 Sequence: MTSPSSSPVFRLETLDGGQEDGSEADRGKLDFGSGLPPMESQFQGEDRKFAPQIRVNLNYRKGTGASQPDPNRFDRDRLFNAVSRGVPEDLAGLPEYLSKTSKYLTDSEYTEGSTGKTCLMKAVLNLKDGVNACILPLLQIDRDSGNPQPLVNAQCTDDYYRGHSALHIAIEKRSLQCVKLLVENGANVHARACGRFFQKGQGTCFYFGELPLSLAACTKQWDVVSYLLENPHQPASLQATDSQGNTVLHALVMISDNSAENIALVTSMYDGLLQAGARLCPTVQLEDIRNLQDLTPLKLAAKEGKIEIFRHILQREFSGLSHLSRKFTEWCYGPVRVSLYDLASVDSCEE Predict reactive species |
| Full Name | transient receptor potential cation channel, subfamily V, member 2 |
| Calculated Molecular Weight | 764 aa, 86 kDa |
| Observed Molecular Weight | 90 kDa |
| GenBank Accession Number | BC018926 |
| Gene Symbol | TRPV2 |
| Gene ID (NCBI) | 51393 |
| RRID | AB_3085477 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y5S1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Transient receptor potential vanilloid 2 (TRPV2), also name as VRL-1, plays a critical role in neuronal development, cardiac function, immunity, and cancer (PMID: 31566564). It has been found to play an essential role in sustained intracellular Ca2+ concentration increase (PMID: 31857697). It's reported that TRPV2 is activated by high temperatures, with a threshold of around 52℃ (PMID: 10201375). The calculated molecular of TRPV2 is 86 kDa, 15991-1-AP can detect the band around 90 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TRPV2 antibody 15991-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TRPV2 Polyclonal antibody (catalog 15991-1-AP) has 6 total citations. These appear in EMBO Molecular Medicine, American Journal of Physiology—Heart and Circulatory Physiology, Herz, Bioscience, Biotechnology, and Biochemistry, Stem Cell Research, and Research Square. The research spans inflammasome/NLRP3-mediated disease, cardiac calcium signaling and myocardial ischemia/reperfusion injury, skeletal muscle TRP channel function, TRPV2 knockout stem cell modeling, and endothelial-to-mesenchymal transition in pulmonary hypertension. Most studies applied the antibody for Western blot in human, mouse, and rat samples.
| Species | Application | Title |
|---|---|---|
Am J Physiol Heart Circ Physiol Effect of long-term heat strain on calcium signaling in the murine myocardium. | ||
Herz Tetrandrine has protective role in myocardial ischemia/reperfusion injury via the TRPV2/Ca(2+)/calcineurin/NFAT axis. | ||
Biosci Biotechnol Biochem Effects of transient receptor potential channel activation during myogenic progression and skeletal muscle function during voluntary exercise. | ||
Stem Cell Res Establishment of a TRPV2 knockout human embryonic stem cell line (WAe009-A-1Y) using episomal vector-based CRISPR/Cas9
| ||
Res Sq Poly-Pharmacologic Disruption of the Proliferative-to-Mesenchymal Fate Branch Point Reverses EndMT and Pulmonary Hypertension. |
