Tested Applications
| Positive WB detected in | human heart tissue, HepG2 cells, K-562 cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16017-1-AP targets ILKAP in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8826 Product name: Recombinant human ILKAP protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 1-392 aa of BC006576 Sequence: MDLFGDLPEPERSPRPAAGKEAQKGPLLFDDLPPASSTDSGSGGPLLFDDLPPASSGDSGSLATSISQMVKTEGKGAKRKTSEEEKNGSEELVEKKVCKASSVIFGLKGYVAERKGEREEMQDAHVILNDITEECRPPSSLITRVSYFAVFDGHGGIRASKFAAQNLHQNLIRKFPKGDVISVEKTVKRCLLDTFKHTDEEFLKQASSQKPAWKDGSTATCVLAVDNILYIANLGDSRAILCRYNEESQKHAALSLSKEHNPTQYEERMRIQKAGGNVRDGRVLGVLEVSRSIGDGQYKRCGVTSVPDIRRCQLTPNDRFILLACDGLFKVFTPEEAVNFILSCLEDEKIQTREGKSAADARYEAACNRLANKAVQRGSADNVTVMVVRIGH Predict reactive species |
| Full Name | integrin-linked kinase-associated serine/threonine phosphatase 2C |
| Calculated Molecular Weight | 392 aa, 43 kDa |
| Observed Molecular Weight | 43-45 kDa |
| GenBank Accession Number | BC006576 |
| Gene Symbol | ILKAP |
| Gene ID (NCBI) | 80895 |
| RRID | AB_2249188 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H0C8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ILKAP antibody 16017-1-AP | Download protocol |
| WB protocol for ILKAP antibody 16017-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
ILKAP Polyclonal antibody (Cat# 16017-1-AP) has a total of 2 citations, published in Frontiers in Medicine and Experimental Eye Research. The associated research fields include hepatocellular carcinoma progression with PGAM1-mediated glycolytic reprogramming, and uveal melanoma progression linked to RNA pseudouridylation. Cited applications are Western blot and immunohistochemistry in human samples.
| Species | Application | Title |
|---|---|---|
Front Med ILKAP drives hepatocellular carcinoma progression by modulating PGAM1-mediated glycolytic reprogramming.
| ||
Exp Eye Res Single-cell transcriptomics reveals that RNA pseudouridylation-related gene NHP2 promotes the progression of uveal melanoma and validated in vivo and in vitro |
