Tested Applications
| Positive WB detected in | Ramos cells, HepG2 cells, Jurkat cells, HEK-293T cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells, Caco-2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16082-1-AP targets PCIF1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9080 Product name: Recombinant human PCIF1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 355-704 aa of BC013365 Sequence: DSVEGICSKIYHISLEYVKRIREKHLAILKENNISEEVEAPEVEPRLVYCYPVRLAVSAPPMPSVEMHMENNVVCIRYKGEMVKVSRNYFSKLWLLYRYSCIDDSAFERFLPRVWCLLRRYQMMFGVGLYEGTGLQGSLPVHVFEALHRLFGVSFECFASPLNCYFRQYCSAFPDTDGYFGSRGPCLDFAPLSGSFEANPPFCEELMDAMVSHFERLLESSPEPLSFIVFIPEWREPPTPALTRMEQSRFKRHQLILPAFEHEYRSGSQHICKKEEMHYKAVHNTAVLFLQNDPGFAKWAPTPERLQELSAAYRQSGRSHSSGSSSSSSSEAKDRDSGREQGPSREPHPT Predict reactive species |
| Full Name | PDX1 C-terminal inhibiting factor 1 |
| Calculated Molecular Weight | 704 aa, 81 kDa |
| Observed Molecular Weight | 81 kDa |
| GenBank Accession Number | BC013365 |
| Gene Symbol | PCIF1 |
| Gene ID (NCBI) | 63935 |
| RRID | AB_2159996 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H4Z3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PCIF1 antibody 16082-1-AP | Download protocol |
| IHC protocol for PCIF1 antibody 16082-1-AP | Download protocol |
| WB protocol for PCIF1 antibody 16082-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-PCIF1 (Cat# 16082-1-AP) has 7 citations across high-impact journals including Nature Communications, Molecular Cell, PNAS, EMBO Journal, NPJ Precision Oncology, Cell Reports, and Journal of Virology. Research fields span RNA m⁶A methylation, virology (HIV, alphaherpesvirus, pseudorabies virus), oncology (glioma, colorectal cancer), SARS-CoV-2 susceptibility, and anti-PD-1 immunotherapy. Applications include WB, IP, and IF in human, mouse, and pig samples.
| Species | Application | Title |
|---|---|---|
Nat Commun HIV reprograms host m 6 Am RNA methylome by viral Vpr protein-mediated degradation of PCIF1 | ||
Mol Cell RNA Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits | ||
NPJ Precis Oncol The WW domain presents a promising target for the development of PCIF1 agonists in the treatment of glioma. | ||
Proc Natl Acad Sci U S A PCIF1-mediated deposition of 5'-cap N6,2'-O-dimethyladenosine in ACE2 and TMPRSS2 mRNA regulates susceptibility to SARS-CoV-2 infection | ||
EMBO J Role of PCIF1-mediated 5\'-cap N6-methyladeonsine mRNA methylation in colorectal cancer and anti-PD-1 immunotherapy | ||
Cell Rep Alphaherpesvirus US3 protein-mediated inhibition of the m6A mRNA methyltransferase complex. |
Reviews
What customers say
One reviewer rated this antibody 3 out of 5 for Western Blot, testing it on 786-O and 769p cell lines. The protocol used a 1-hour block in 5% milk/TBST, primary antibody at 1/1000 in 3% BSA/TBST overnight at 4°C, and secondary antibody at 1/5000 in 5% milk/TBST. The moderate rating suggests acceptable but not outstanding performance under these conditions.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Anirban (Verified Customer) (01-25-2023) | blocking 1 hr in 5% milk/TBST. Primary ab- 1/1000 in 3% BSA/TBST at 4 deg for 16 hrs, Secondary ab-1/5000 in 5% milk/TBST.
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