Tested Applications
| Positive WB detected in | mouse spleen tissue, mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16095-1-AP targets ASB1 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8848 Product name: Recombinant human ASB1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-335 aa of BC014528 Sequence: MAEGGSPDGRAGPGSAGRNLKEWLREQFCDHPLEHCEDTRLHDAAYVGDLQTLRSLLQEESYRSRINEKSVWCCGWLPCTPLRIAATAGHGSCVDFLIRKGAEVDLVDVKGQTALYVAVVNGHLESTQILLEAGADPNGSRHHRSTPVYHASRVGRADILKALIRYGADVDVNHHLTPDVQPRFSRRLTSLVVCPLYISAAYHNLQCFRLLLLAGANPDFNCNGPVNTQGFYRGSPGCVMDAVLRHGCEAAFVSLLVEFGANLNLVKWESLGPESRGRRKVDPEALQVFKEARSVPRTLLCLCRVAVRRALGKHRLHLIPSLPLPDPIKKFLLHE Predict reactive species |
| Full Name | ankyrin repeat and SOCS box-containing 1 |
| Calculated Molecular Weight | 335 aa, 37 kDa |
| Observed Molecular Weight | 37 kDa |
| GenBank Accession Number | BC014528 |
| Gene Symbol | ASB1 |
| Gene ID (NCBI) | 51665 |
| RRID | AB_2918043 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y576 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ASB1 antibody 16095-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-ASB1 antibody (Cat# 16095-1-AP) has 2 total citations. They appear in Redox Biology and Emerging Microbes & Infection. The research fields span redox biology and reproductive biology (H₂S homeostasis during spermiogenesis) and virology/immunology (K63-linked polyubiquitination of MAVS restricting influenza A virus replication). Applications include WB and IP in mouse and human samples.
| Species | Application | Title |
|---|---|---|
Redox Biol ASB1 engages with ELOB to facilitate SQOR ubiquitination and H2S homeostasis during spermiogenesis | ||
Emerg Microbes Infect ATG13 promotes K63-linked polyubiquitination of MAVS via ASB1 to restrict influenza A virus replication.
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