Tested Applications
| Positive WB detected in | mouse lung tissue |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16097-1-AP targets PELI2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8940 Product name: Recombinant human PELI2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 281-420 aa of BC009476 Sequence: RPQCPVGLNTLAFPSINRKEVVEEKQPWAYLSCGHVHGYHNWGHRSDTEANERECPMCRTVGPYVPLWLGCEAGFYVDAGPPTHAFTPCGHVCSEKSAKYWSQIPLPHGTHAFHAACPFCATQLVGEQNCIKLIFQGPID Predict reactive species |
| Full Name | pellino homolog 2 (Drosophila) |
| Calculated Molecular Weight | 420 aa, 46 kDa |
| Observed Molecular Weight | 45-50 kDa |
| GenBank Accession Number | BC009476 |
| Gene Symbol | PELI2 |
| Gene ID (NCBI) | 57161 |
| RRID | AB_2878218 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HAT8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Pellino2 (PELI2) is a newly discovered E3 ubiquitin ligase, which regulates the protein degradation, protein-protein interaction, protein translocation and signaling transduction via the ubiquitination of target proteins, and plays important roles in inflammation and the immune system. PELI2 possesses a C-terminal RING-like domain and a phospho-threonine-binding forkhead-associated (FHA) domain that are responsible for ubiquitin ligase activity and substrate binding, respectively (PMID: 24699078).IHC
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PELI2 antibody 16097-1-AP | Download protocol |
| WB protocol for PELI2 antibody 16097-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PELI2 Polyclonal antibody (Cat# 16097-1-AP) has 1 citation. It appears in Molecular Medicine (IF 8.3), published in 2025. The study investigated how PELI2 inhibits colorectal cancer development through the MAPK signaling pathway, with the antibody applied in WB and IHC on human samples. The research falls within the fields of oncology and signal transduction.
| Species | Application | Title |
|---|---|---|
