Tested Applications
| Positive WB detected in | mouse liver tissue, rat liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16234-1-AP targets FOXS1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8957 Product name: Recombinant human FOXS1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-330 aa of BC013408 Sequence: MQQQPLPGPGAPTTEPTKPPYSYIALIAMAIQSSPGQRATLSGIYRYIMGRFAFYRHNRPGWQNSIRHNLSLNECFVKVPRDDRKPGKGSYWTLDPDCHDMFEHGSFLRRRRRFTRQTGAEGTRGPAKARRGPLRATSQDPGVPNATTGRQCSFPPELPDPKGLSFGGLVGAMPASMCPATTDGRPRPPMEPKEISTPKPACPGELPVATSSSSCPAFGFPAGFSEAESFNKAPTPVLSPESGIGSSYQCRLQALNFCMGADPGLEHLLASAAPSPAPPTPPGSLRAPLPLPTDHKEPWVAGGFPVQGGSGYPLGLTPCLYRTPGMFFFE Predict reactive species |
| Full Name | forkhead box S1 |
| Calculated Molecular Weight | 330 aa, 35 kDa |
| Observed Molecular Weight | 35 kDa |
| GenBank Accession Number | BC013408 |
| Gene Symbol | FOXS1 |
| Gene ID (NCBI) | 2307 |
| RRID | AB_2878234 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43638 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FOXS1 antibody 16234-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The FOXS1 antibody (catalog number 16234-1-AP) has 3 total citations. These appear in International Immunopharmacology, FASEB Journal, and Journal of Biological Chemistry. The associated research fields include renal fibrosis and epithelial-mesenchymal transition (EMT) via the FOXS1/Wnt/β-catenin pathway, FOXS1 as an oncogene inducing EMT in prostate cancer, and TGF-β1-dependent FOXS1 expression affecting adipogenic and myofibroblast phenotypes.
| Species | Application | Title |
|---|---|---|
Int Immunopharmacol Adipose mesenchymal stem cell-derived extracellular vesicles alleviate renal fibrosis by reducing epithelial-mesenchymal transition via the FOXS1/Wnt/β-catenin signaling pathway
| ||
FASEB J FOXS1 acts as an oncogene and induces EMT through FAK/PI3K/AKT pathway by upregulating HILPDA in prostate cancer | ||
J Biol Chem TGF-β1-dependent expression of FOXS1 attenuates adipogenic potential and enhances a myofibroblast cellular phenotype |
Reviews
What customers say
The single review for this antibody is a 5-star rating from a user who tested it for immunohistochemistry and immunofluorescence. The reviewer reports it works extremely well with overnight incubation at 1:250–1:500 dilution in 1x TBST containing 5% donkey serum, using human-derived tissue and cells. Specifically, a 1:250 dilution was used for 3D PFA-fixed tissue and 1:500 for PFA-fixed 2D cells. Overall, the feedback is strongly positive, highlighting reliable performance across both applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Refik (Verified Customer) (10-27-2025) | Works extremely well with overnight incubation at 1:250-500 dilution in 1x TBST in 5% Donkey serum in human derived tissue and cells
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