Tested Applications
| Positive WB detected in | mouse lung tissue, mouse ovary tissue |
| Positive IP detected in | mouse lung tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16252-1-AP targets DMKN in WB, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9242 Product name: Recombinant human DMKN protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 334-476 aa of BC011886 Sequence: SETSPGMFNFDTFWKNFKSKLGFINWDAINKNQVPPPSTRALLYFSRLWEDFKQNTPFLNWKAIIEGADASSLQKRAGRADQPGAGWQEVAAVTSKNYNYNQHAYPTAYGGKYSVKTPAKGGVSPSSSASRVQPGLLQWVKFW Predict reactive species |
| Full Name | dermokine |
| Calculated Molecular Weight | 476 aa, 47 kDa |
| Observed Molecular Weight | 48-55 kDa |
| GenBank Accession Number | BC011886 |
| Gene Symbol | DMKN |
| Gene ID (NCBI) | 93099 |
| RRID | AB_10596473 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6E0U4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DMKN, also named as epidermis-specific secreted protein SK30/SK89, acts as a soluble regulator of keratinocyte differentiation. DMKN has 16 isoforms with MW 9-20 kDa, 35-38 kDa and 42-48 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for DMKN antibody 16252-1-AP | Download protocol |
| WB protocol for DMKN antibody 16252-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The DMKN polyclonal antibody (Cat# 16252-1-AP) has 2 total citations, both published in Molecular & Cellular Proteomics. The cited studies focus on proteomics and degradomics research in skin biology—one investigating matrix metalloproteinase 10 substrates in keratinocytes and epidermal tissue, and the other examining the impact of cysteine cathepsin B/L deletion on the murine skin proteome.
| Species | Application | Title |
|---|---|---|
Mol Cell Proteomics Deletion of cysteine cathepsins B or L yields differential impacts on murine skin proteome and degradome. | ||
Mol Cell Proteomics Matrix metalloproteinase 10 degradomics in keratinocytes and epidermal tissue identifies bioactive substrates with pleiotropic functions. |
