Tested Applications
| Positive WB detected in | HeLa cells, U-937 cells, mouse kidney tissue, mouse liver tissue, human brain tissue, HepG2 cells, HEK-293 cells, Jurkat cells |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | mouse small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16257-1-AP targets GGCT in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9284 Product name: Recombinant human GGCT protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-188 aa of BC019243 Sequence: MANSGCKDVTGPDEESFLYFAYGSNLLTERIHLRNPSAAFFCVARLQDFKLDFGNSQGKTSQTWHGGIATIFQSPGDEVWGVVWKMNKSNLNSLDEQEGVKSGMYVVIEVKVATQEGKEITCRSYLMTNYESAPPSPQYKKIICMGAKENGLPLEYQEKLKAIEPNDYTGKVSEEIEDIIKKGETQTL Predict reactive species |
| Full Name | gamma-glutamyl cyclotransferase |
| Calculated Molecular Weight | 188 aa, 21 kDa |
| Observed Molecular Weight | 21-25 kDa |
| GenBank Accession Number | BC019243 |
| Gene Symbol | GGCT |
| Gene ID (NCBI) | 79017 |
| RRID | AB_10852962 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75223 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GGCT antibody 16257-1-AP | Download protocol |
| IHC protocol for GGCT antibody 16257-1-AP | Download protocol |
| IP protocol for GGCT antibody 16257-1-AP | Download protocol |
| WB protocol for GGCT antibody 16257-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GGCT Polyclonal antibody (Cat# 16257-1-AP) has 10 citations published in journals including Redox Biology, iScience, FASEB Journal, Scientific Reports, Cancer Gene Therapy, International Journal of Molecular Sciences, Biochimica et Biophysica Acta, Cancer Science, Journal of Cancer Research and Clinical Oncology, and Cancer Genomics Proteomics. Research fields span cancer biology (glioblastoma, thyroid, osteosarcoma, breast, and pancreatic cancers), redox/oxidative stress, metabolic disorders, and atherosclerosis.
| Species | Application | Title |
|---|---|---|
Redox Biol Unbalanced redox status network as an early pathological event in congenital cataracts | ||
Cancer Gene Ther γ-Glutamylcyclotransferase is transcriptionally regulated by c-Jun and controls proliferation of glioblastoma stem cells through Notch1 levels
| ||
Int J Mol Sci Interaction of MRPL9 and GGCT Promotes Cell Proliferation and Migration by Activating the MAPK/ERK Pathway in Papillary Thyroid Cancer | ||
Biochim Biophys Acta Mol Basis Dis Integrated application of transcriptome and metabolomics reveals potential therapeutic targets for the polarization of atherosclerotic macrophages | ||
Cancer Sci Myc upregulates Ggct, γ-glutamylcyclotransferase to promote development of p53-deficient osteosarcoma | ||
Sci Rep Identification of proteins responsible for adriamycin resistance in breast cancer cells using proteomics analysis. |
