Tested Applications
| Positive WB detected in | HL-60 cells, K-562 cells, human placenta tissue |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16335-1-AP targets ATP5S in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9486 Product name: Recombinant human ATP5S protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-127 aa of BC011549 Sequence: MCCAVSEQRLTCADQMMLFGKISQQLCGVKKLPWSCDSRYFWGWLNAVFNKVDYDRIRDVGPDRAASEWLLRCGAMVRYHGQERWQKDYNHLPTGPLDKYKIQAIDATDSCIMSIGFDHMETSNICC Predict reactive species |
| Full Name | ATP synthase, H+ transporting, mitochondrial F0 complex, subunit s (factor B) |
| Calculated Molecular Weight | 127aa,15 kDa; 215aa,25 kDa |
| Observed Molecular Weight | 23 kDa |
| GenBank Accession Number | BC011549 |
| Gene Symbol | ATP5S |
| Gene ID (NCBI) | 27109 |
| RRID | AB_3741884 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99766 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DMAC2L also known as ATP5S, belongs to the ATP synthase family, which is a group of enzymes that play a crucial role in cellular energy production. ATP synthase is the central enzyme in oxidative phosphorylation, responsible for the production of most of the ATP in mammalian organisms. ATP5S is the S subunit (also known as factor B) of mitochondrial FO complex of ATP synthase. It is an essential component of the H+ channel of the FO complex (PMID:7706317, 6143319).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ATP5S antibody 16335-1-AP | Download protocol |
| WB protocol for ATP5S antibody 16335-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ATP5S Polyclonal antibody (Cat# 16335-1-AP) has 1 citation. It appears in Biochemical Genetics (2015), in a study investigating how miR-34a mediates resistin-induced ATP depletion by targeting ATP5S in HepG2 hepatocellular carcinoma cells. The associated research fields include microRNA regulation, cellular energy metabolism (ATP homeostasis), and liver cancer biology. The antibody was validated by Western blot in human samples.
| Species | Application | Title |
|---|---|---|
Biochem Genet MiR-34a is Involved in the Decrease of ATP Contents Induced by Resistin Through Target on ATP5S in HepG2 Cells. |
