Tested Applications
| Positive WB detected in | Jurkat cells, HeLa cells, human testis tissue |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16501-1-AP targets DNAJB14 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9668 Product name: Recombinant human DNAJB14 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-254 aa of BC022248 Sequence: MEGNRDEAEKCVEIAREALNAGNREKAQRFLQKAEKLYPLPSARALLEIIMKNGSTAGNSPHCRKPSGSGDQSKPNCTKDSTSGSGEGGKGYTKDQVDGVLSINKCKNYYEVLGVTKDAGDEDLKKAYRKLALKFHPDKNHAPGATDAFKKIGNAYAVLSNPEKRKQYDLTGNEEQACNHQNNGRFNFHRGCEADITPEDLFNIFFGGGFPSGSVHSFSNGRAGYSQQHQHRHSGHEREEERGDGGFSVFIQLM Predict reactive species |
| Full Name | DnaJ (Hsp40) homolog, subfamily B, member 14 |
| Calculated Molecular Weight | 379 aa, 43 kDa |
| Observed Molecular Weight | 43 kDa |
| GenBank Accession Number | BC022248 |
| Gene Symbol | DNAJB14 |
| Gene ID (NCBI) | 79982 |
| RRID | AB_2094414 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8TBM8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for DNAJB14 antibody 16501-1-AP | Download protocol |
| IHC protocol for DNAJB14 antibody 16501-1-AP | Download protocol |
| IP protocol for DNAJB14 antibody 16501-1-AP | Download protocol |
| WB protocol for DNAJB14 antibody 16501-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The DNAJB14 polyclonal antibody (Cat# 16501-1-AP) has 10 citations. These appear in journals including Nature Communications, PLoS Pathogens, Journal of Virology, FEBS Journal, Molecular Biology of the Cell, Molecular and Cellular Biochemistry, and eLife. The research fields span non-enveloped virus ER exit mechanisms, ER membrane J-protein/chaperone functions, selective autophagy, and mitochondrial stress responses. Cited applications include WB and IF in human, mouse, and monkey systems.
| Species | Application | Title |
|---|---|---|
Nat Commun Selective EMC subunits act as molecular tethers of intracellular organelles exploited during viral entry. | ||
PLoS Pathog A Non-enveloped Virus Hijacks Host Disaggregation Machinery to Translocate across the Endoplasmic Reticulum Membrane. | ||
PLoS Pathog A cytosolic chaperone complexes with dynamic membrane J-proteins and mobilizes a nonenveloped virus out of the endoplasmic reticulum. | ||
Mol Cell Biochem Novel functions of the ER-located Hsp40s DNAJB12 and DNAJB14 on proteins at the outer mitochondrial membrane under stress mediated by CCCP | ||
FEBS J. Hydrophobic domains of mouse polyomavirus minor capsid proteins promote membrane association and virus exit from the ER. | ||
J Virol The ER membrane J protein C18 executes a distinct role in promoting SV40 membrane penetration. |
