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ARH3 Polyclonal antibody

ARH3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 16504-1-AP
Publications (2)
Review Product
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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

ADP-ribosylhydrolase 3, ADP-ribose glycohydrolase ARH3, ADPRHL2, ADPRHL 2, [Protein ADP-ribosylserine] hydrolase

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
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  • Western Blot (WB) analysis of various lysates using ARH3 Polyclonal antibody (16504-1-AP)

    WB analysis using 16504-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16504-1-AP (ARH3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Western Blot (WB) analysis of HeLa cells using ARH3 Polyclonal antibody (16504-1-AP)

    WB analysis of HeLa using 16504-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16504-1-AP (ARH3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Western Blot (WB) analysis of mouse brain tissue using ARH3 Polyclonal antibody (16504-1-AP)

    WB analysis of mouse brain using 16504-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 16504-1-AP (ARH3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Western Blot (WB) analysis of HepG2 cells using ARH3 Polyclonal antibody (16504-1-AP)

    WB analysis of HepG2 using 16504-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16504-1-AP (ARH3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Western Blot (WB) analysis of mouse heart tissue using ARH3 Polyclonal antibody (16504-1-AP)

    WB analysis of mouse heart using 16504-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 16504-1-AP (ARH3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Western Blot (WB) analysis of mouse lung tissue using ARH3 Polyclonal antibody (16504-1-AP)

    WB analysis of mouse lung using 16504-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 16504-1-AP (ARH3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Western Blot (WB) analysis of mouse kidney tissue using ARH3 Polyclonal antibody (16504-1-AP)

    WB analysis of mouse kidney using 16504-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16504-1-AP (ARH3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Western Blot (WB) analysis of mouse thymus tissue using ARH3 Polyclonal antibody (16504-1-AP)

    WB analysis of mouse thymus using 16504-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 16504-1-AP (ARH3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunohistochemistry (IHC) staining of mouse testis tissue using ARH3 Polyclonal antibody (16504-1-AP)

    IHC staining of mouse testis using 16504-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16504-1-AP (ARH3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunohistochemistry (IHC) staining of mouse testis tissue using ARH3 Polyclonal antibody (16504-1-AP)

    IHC staining of mouse testis using 16504-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16504-1-AP (ARH3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunohistochemistry (IHC) staining of mouse brain tissue using ARH3 Polyclonal antibody (16504-1-AP)

    IHC staining of mouse brain using 16504-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16504-1-AP (ARH3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunohistochemistry (IHC) staining of mouse brain tissue using ARH3 Polyclonal antibody (16504-1-AP)

    IHC staining of mouse brain using 16504-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16504-1-AP (ARH3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ARH3 Polyclonal antibody (16504-1-AP)

    IF Staining of HepG2 using 16504-1-AP

    Immunofluorescent analysis of HepG2 cells using 16504-1-AP (ARH3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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Tested Applications

Positive WB detected inmouse brain tissue, HeLa cells, HepG2 cells, mouse heart tissue, mouse lung tissue, mouse kidney tissue, mouse thymus tissue, rat brain tissue
Positive IHC detected inmouse testis tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16504-1-AP targets ARH3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag9683

Product name: Recombinant human ADPRHL2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-363 aa of BC014169

Sequence: MAAAAMAAAAGGGAGAARSLSRFRGCLAGALLGDCVGSFYEAHDTVDLTSVLRHVQSLEPDPGTPGSERTEALYYTDDTAMARALVQSLLAKEAFDEVDMAHRFAQEYKKDPDRGYGAGVVTVFKKLLNPKCRDVFEPARAQFNGKGSYGNGGAMRVAGISLAYSSVQDVQKFARLSAQLTHASSLGYNGAILQALAVHLALQGESSSEHFLKQLLGHMEDLEGDAQSVLDARELGMEERPYSSRLKKIGELLDQASVTREEVVSELGNGIAAFESVPTAIYCFLRCMEPDPEIPSAFNSLQRTLIYSISLGGDTDTIATMAGAIAGAYYGMDQVPESWQQSCEGYEETDILAQSLHRVFQKS

Predict reactive species
Full Name ADP-ribosylhydrolase like 2
Calculated Molecular Weight 363 aa, 39 kDa
Observed Molecular Weight 39 kDa
GenBank Accession NumberBC014169
Gene Symbol ARH3
Gene ID (NCBI) 54936
RRIDAB_2224919
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NX46
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARH3 can be detected as two bands between 39 and 40 kDa.(PMID: 16278211/32636369)

Protocols

Product Specific Protocols
IF protocol for ARH3 antibody 16504-1-APDownload protocol
IHC protocol for ARH3 antibody 16504-1-APDownload protocol
WB protocol for ARH3 antibody 16504-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The ARH3 Polyclonal antibody (Cat# 16504-1-AP) has 2 citations. One appears in Nature Communications (IF 18.1), studying cis- and trans-resveratrol effects on histone ADP-ribosylation and tyrosine-induced neurodegeneration in human and rat samples via WB and IP. The other is in Molecular Cell (IF 16), investigating deacetylated PCBP1 licensing of PARP1 activity for DNA damage repair in human cells via WB. The associated research fields are neurodegeneration and DNA damage repair.

Automatically generated from the 2 publication citations by Able AI.
SpeciesApplicationTitle
human,ratWB,IP

Nat Commun

Cis- and trans-resveratrol have opposite effects on histone serine-ADP-ribosylation and tyrosine induced neurodegeneration.

Authors - Megha Jhanji
View Article
humanWB

Mol Cell

Deacetylated PCBP1 licenses PARP1 activity for DNA damage repair

Authors - Yuxin Shu
View Article
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